The Transient Receptor Potential Channel Vanilloid 1 (TRPV1) is Critical in Innate Airway Epithelial Responses to Protease Allergens.

The Transient Receptor Potential Channel Vanilloid 1 (TRPV1) is Critical in Innate Airway Epithelial Responses to Protease Allergens. Am J Respir Cell Mol Biol. 2020 Mar 17;: Authors: Schiffers C, Hristova M, Habibovic A, Dustin CM, Danyal K, Reynaert NL, Wouters EFM, van der Vliet A Abstract The airway epithelium plays a critical role in innate responses to airborne allergens by secreting IL-1 family cytokines such as interleukin (IL)-1α and IL-33, as alarmins that subsequently orchestrate appropriate immune responses. Previous studies revealed that epithelial IL-33 secretion by allergens such as A. alternaria (ALT) or house dust mite (HDM) involves Ca2+-dependent signaling, via initial activation of ATP-stimulated type 2 purinoceptors (P2YR2) and subsequent activation of the NADPH oxidase DUOX1. We sought to identify proximal mechanisms by which epithelial cells sense these allergens, and here highlight the importance of protease-activated receptors (PAR2) and transient receptor potential (TRP) Ca2+ channels such as TRP Vanilloid 1 (TRPV1) in these responses. Combined studies of primary human nasal and mouse tracheal epithelial cells, as well as immortalized human bronchial epithelial cells (HBE1), indicated the importance of both PAR2 and TRPV1 in IL-33 secretion by both ALT and HDM, based on both pharmacological and genetic approaches. TRPV1 was also critically involved in allergen-induced ATP release, activation of DUOX1, and r...
Source: Am J Respir Cell Mol... - Category: Respiratory Medicine Authors: Tags: Am J Respir Cell Mol Biol Source Type: research