CircPRKCI relieves lipopolysaccharide ‐induced HK2 cell injury by upregulating the expression of miR‐545 target gene ZEB2

AbstractThe aim of this study was to investigate the possible influences of circPRKCI abnormal expression on lipopolysaccharide (LPS) ‐induced HK2 cell injury and its mechanism. The circPRKCI level was identified in serum samples from patients with urosepsis and healthy subjects, as well as LPS‐treated HK2 cells by qRT‐PCR. Cell viability, apoptosis, expression of proteins associated with apoptosis, and expression of pro‐i nflammatory cytokines in LPS‐treated HK2 cells were measured. Effects of circPRKCI abnormal expression on LPS‐induced HK2 cell injury were then evaluated. Afterward, the binding miRNA of circPRKCI and target gene of miRNA were identified, and the involvements of NF‐kB pathway signaling pathway with the effects of circPRKCI were finally studied. CircPRKCI was significantly down‐regulated in serum samples from patients with urosepsis and LPS‐treated HK2 cells. LPS‐induced decrease of cell viability, increase of cell apoptosis, as well as elevated productions of tumor necrosis factor (TNF)‐α, interleukins (IL)‐1β, IL‐6, and IL‐8 in HK2 cells were attenuated by overexpressed circPRKCI. In addition, circPRKCI negatively regulated the expression of miR‐545, and miR‐545 up‐regulation reversed the inhibiting effects of circPRKCI overexpression on LPS‐induced HK2 cel l injury. Moreover, zinc finger E‐box‐binding homeobox 2 (ZEB2) was identified as a target gene of miR‐545, and ZEB2 overexpression partly reversed the effect...

Source: BioFactors - March 4, 2020 Category: Biochemistry Authors: Xiaofeng Shi, Wei Ma, Yongqi Li, Han Wang, Shuang Pan, Yongquan Pan, Caiming Xu, Lei Li Tags: Research Communication Source Type: research