Design of organic/inorganic nanocomposites for ultrasensitive electrochemical detection of a cancer biomarker protein.

Design of organic/inorganic nanocomposites for ultrasensitive electrochemical detection of a cancer biomarker protein. Talanta. 2020 May 15;212:120794 Authors: Zhang X, Yu Y, Shen J, Qi W, Wang H Abstract A new type of nanocomposite composed of carboxylated single-walled carbon nanotubes (CNTs-COOH), reduced graphene oxide (rGO), bovine serum albumin-Ag hybride (Ag@BSA), and poly(3,4-ethylenedioxythiophene) (PEDOT) was fabricated to develop an ultrasensitive electrochemical platform for the detection of carcinoembryonic antigen (CEA) as a model of biomarkers. Two steps are involved for the fabrication of the organic/inorganic nanocomposites. The Ag@BSA nanoflowers were first synthesized to be doped with CNTs-COOH and rGO followed by the adsorption of PEDOT resulting in CNTs-COOH/rGO/Ag@BSA/PEDOT. The as-prepared nanocomposites were then deposited onto an Au electrode together with subsequent immobilization of CEA antibody (anti-CEA) to construct the electrochemical immunosensor. This unique structure and composition of the developed immunosensor can expect an excellent electrochemical response. The immunosensor offers a linear relationship between the electrochemical responses and the CEA concentrations from 0.002 to 50 ng∙mL-1 with a detection limit of 1 × 10-4 ng∙mL-1. Moreover, the ultrasensitive immunoassay can detect CEA in real human serum samples, and the results are comparable to those obtained from the commercial EL...
Source: Talanta - Category: Chemistry Authors: Tags: Talanta Source Type: research