Systematic optimization of square-wave electroporation conditions for bovine primary fibroblasts

Gene transfer by electroporation is an established method for the non-viral mediated transfection of mammalian cells. Primary cells pose a particular challenge for electroporation-mediated gene transfer, since...

Source: BMC Cell Biology - February 28, 2020 Category: Cytology Authors: Iqbal Hyder, Shahin Eghbalsaied and Wilfried A. Kues Tags: Methodology article Source Type: research