CyTOF-based Whole Blood Assay to Explore the Allergen Specific Signaling Pathways in Basophil

Peanut allergy is the most common cause of anaphylaxis in childhood. This IgE-mediated allergic reaction is initiated by basophils or mast cells upon exposure and cross-linking of membrane bound IgE by allergens. The subtle molecular and cellular mechanisms that define the difference between peanut allergy and asymptomatic sensitization and during oral immunotherapy are not fully understood. We established a mass cytometry (CyTOF) panel to study differences in allergen specific signaling pathways upon peanut stimulation in human whole blood.
Source: Journal of Allergy and Clinical Immunology - Category: Allergy & Immunology Authors: Source Type: research