Epidemiological analysis of Arcanobacterium phocae isolated from cases of mink dermatitis of a single farm

Publication date: Available online 22 February 2020Source: Veterinary MicrobiologyAuthor(s): Mazen Alssahen, Abdulwahed Ahmed Hassan, Osama Sammra, Christoph Lämmler, Mirja Raunio Saarnisto, Maria Borowiak, Burkhard Malorny, Jörg Rau, Ellen Prenger-Berninghoff, Madelein Plötz, Amir AbdulmawjoodAbstractThe present study was designed to identify nine Arcanobacterium phocae strains isolated from cases of mink dermatitis of a single farm in Finland and characterize the strains for epidemiological relationships. All nine strains and previously described A. phocae used for comparative purposes were identified and further characterized phenotypically, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), by Fourier Transform Infrared Spectroscopy (FT-IR) and genotypically by detection of phocaelysin encoding gene phl with a previously developed loop-mediated isothermal amplification (LAMP) assay and by sequencing 16S rRNA gene and gene phl, the elongation factor tu encoding gene tuf and the β subunit of bacterial RNA polymerase encoding gene rpoB. Genetic relatedness among isolates was determined using whole-genome single nucleotide polymorphism (wgSNP) analysis. The wgSNP results, partly the MALDI-TOF MS and FT-IR analyses and sequencing of the genes, revealed that the nine A. phocae strains recovered from a single farm showed close sequence similarities among each other and differed from previously investigated A. phocae strains isolat...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research