New combination therapy of gliclazide and quercetin for protection against STZ-induced diabetic rats

Publication date: Available online 21 February 2020Source: Life SciencesAuthor(s): Noha F. Abdelkader, Hebatollah E. Eitah, Yousreya A. Maklad, Amina A. Gamaleldin, Manal A. Badawi, Sanaa A. KenawyAbstractAimsThe use of natural agents with anti-diabetic effect in combination therapy adds further positive clinical implications in the management of diabetes mellitus. Interestingly, quercetin is one of the most potent naturally occurring antioxidant which possesses various pharmacological actions including anti-diabetic effect. Thus, this research was conducted to assess the efficiency of a new combination from gliclazide and quercetin on glycemic control as well as pancreatic islets and beta cells in STZ-experimental model of diabetes.Main methodsDiabetes has been induced by a single intraperitoneal injection of streptozotocin (STZ; 45 mg/kg) in adult male Wistar rats. For 3 consecutive weeks, diabetic rats were given orally either gliclazide (10 mg/kg), quercetin (50 mg/kg), or their combination. At the end of the experiment, histological, immunohistochemical and morphometrical examination of pancreatic tissues was performed. Furthermore, the changes in glucose metabolism, lipid profile, oxidative and inflammatory status were evaluated.Key findingsTreatment with gliclazide alone decreased serum glucose, total cholesterol, triglycerides, malondialdehyde, tumor necrosis factor-alpha and nuclear factor kappa-Beta while increased serum C-peptide, superoxide dismutase, reduce...

Source: Life Sciences - February 21, 2020 Category: Biology Source Type: research