Characterization of Polyurethane Foam Environmental Monitoring Tools for the Recovery and Release of Viruses

This study aims to characterize polyurethane foam (PUF) EM tools —currently used in the food industry for the recovery of bacteria from food contact surfaces—for their efficacy in the release and recovery of human enteric viruses. Two viruses (human norovirus [hNoV] and Tulane virus [TV]) were compared at varying inoculum levels, with two EM tools (PUF swab a nd sponge), two delayed processing times (24 h and 72 h), and one surface type (stainless steel [SS]). Specifically, the objectives were to (1) determine the ability of PUF devices to release viruses for detection and (2) assess the ability of PUF devices to recover viruses from SS surfaces. For T V release from the sponge, there was a significant difference (p = 0.0064) when compared across inoculum level (105 plaque forming unit [PFU]/sponge vs. 102 PFU/sponge). Release of hNoV at a single inoculum level by PUF sponge and swab was compared resulting in a significant difference (p 
Source: Food and Environmental Virology - Category: Virology Source Type: research

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Contributors : Christine Yu ; Kaoru Hida ; Efstathia PapafragkouSeries Type : Expression profiling by genome tiling arrayOrganism : Allium fistulosum ; Apium graveolens ; Astrovirus sp. ; Bacillus subtilis ; Coxsackievirus ; Hepatitis E virus ; Hepatovirus A ; Norovirus ; Rotavirus ; Sapovirus ; Solanum lycopersicum ; unidentified adenovirusThe capability of the U.S. Food and Drug Administration Enteric Viruses tiling microarray (FDA-EVIR) was assessed for rapid molecular identification of human norovirus (NoV) and hepatitis A virus (HAV) extracted from artificially inoculated fresh produce. Two published viral extraction ...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by genome tiling array Allium fistulosum Apium graveolens Astrovirus sp. Bacillus subtilis Coxsackievirus Hepatitis E virus Hepatovirus A Norovirus Rotavirus Sapovirus Solanum lycopersicum unidentified adenovir Source Type: research
(University of Leeds) Every picture tells a story... none more so than this detailed visualisation of a strain of the norovirus. Created from 13,000 separate images taken by an electron microscope, it reveals in rich detail the structure of the virus. It shows bump-like protrusions on the outside of the virus capsid, the protein shell that holds the genome of the virus.
Source: EurekAlert! - Infectious and Emerging Diseases - Category: Infectious Diseases Source Type: news
by Joseph S. Snowden, Daniel L. Hurdiss, Oluwapelumi O. Adeyemi, Neil A. Ranson, Morgan R. Herod, Nicola J. Stonehouse Icosahedral viral capsids must undergo conformational rearrangements to coordinate essential processes during the viral life cycle. Capturing such conformational flexibility has been technically challenging yet could be key for developing rational therapeutic agents to combat infections. Norovirus es are nonenveloped, icosahedral viruses of global importance to human health. They are a common cause of acute gastroenteritis, yet no vaccines or specific antiviral agents are available. Here, we use genetics ...
Source: PLoS Biology: Archived Table of Contents - Category: Biology Authors: Source Type: research
Publication date: Available online 26 March 2020Source: Biosensors and BioelectronicsAuthor(s): Akhilesh Babu Ganganboina, Ankan Dutta Chowdhury, Indra Memdi Khoris, Fahmida Nasrin, Kenshin Takemura, Toshimi Hara, Fuyuki Abe, Tetsuro Suzuki, Enoch Y. Park
Source: Biosensors and Bioelectronics - Category: Biotechnology Source Type: research
In this study, we analyzed the epidemiological and genetic features of NoV outbreaks from July 2014 to June 2018 in Huzhou, China. A total of 450 stool samples collected from 51 AGE outbreaks were tested for NoVs by real-time RT PCR. Partial polymerase and capsid sequences of NoV-positive samples were amplified and sequenced for phylogenetic analysis. NoVs were found to be responsible of 84.3% of AGE outbreaks in Huzhou over the past 5 years. Most NoV outbreaks were reported in the cool months (November-March) and occurred in primary schools and kindergartens. Changes in the diversity of genotypes and the distribution of p...
Source: Archives of Virology - Category: Virology Source Type: research
by Carly Adams, David Young, Paul A. Gasta ñaduy, Prabasaj Paul, Zach Marsh, Aron J. Hall, Benjamin A. Lopman The role of individual case characteristics, such as symptoms or demographics, in norovirus transmissibility is poorly understood. Six nursing home norovirus outbreaks occurring in South Carolina, U.S. from 2014 to 2016 were examined. We aimed to quantify the contribution of symptoms and other cas e characteristics in norovirus transmission using the reproduction number (REi) as an estimate of individual case infectivity and to examine how transmission changes over the course of an outbreak. Individual esti...
Source: PLoS Computational Biology - Category: Biology Authors: Source Type: research
This study provides a framework for designing the appropriate experiments that are likely to lead to accurate and rapid virus emergency response.
Source: Virus Genes - Category: Genetics & Stem Cells Source Type: research
Public health authorities are advising frequent hand washing and social distancing, especially in the absence of confirmatory testing for COVID-19. I don’t have any wisdom to add to these practices. Vaccines are in the works, as are anti-viral drugs—nothing to add here, either. But let me reiterate what we do in the Wheat Belly and Undoctored lifestyles. In general, we do not treat diseases; we correct the factors that allow disease to emerge in the first place—a big difference. Take rheumatoid arthritis, for example. In conventional healthcare, the joint pain and swelling of rheumatoid arthritis are...
Source: Wheat Belly Blog - Category: Cardiology Authors: Tags: Open probiotic undoctored wheat belly Source Type: blogs
Authors: Hanisch FG, Aydogan C Abstract Norovirus infections belong to the most common causes of human gastroenteritis worldwide, and epidemic outbreaks are responsible for hundreds of thousands deaths annually. Strikingly, no antiviral treatment is available due to the difficulty in cultivating virions or in generating a vaccine, and due to the fact that their infection mechanisms are poorly understood. However, there is consent that noroviruses bind to histo-blood group antigens (HBGAs) on their way through the digestive tract. The HBGA profiles vary individually, making people more or less susceptible to differe...
Source: Nestlee Nutrition Institute Workshop Series - Category: Nutrition Tags: Nestle Nutr Inst Workshop Ser Source Type: research
A 61-year-old man lost more than 25 pounds in a matter of a few months and was not responding to treatment. The doctor was stumped.
Source: NYT Health - Category: Consumer Health News Authors: Tags: Immune System Stem Cells Cancer Norovirus Vanderbilt University Medical Center Clinical Infectious Diseases (Journal) Source Type: news
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