Clearance of porcine circovirus and porcine parvovirus from porcine ‐derived pepsin by low pH inactivation and cation exchange chromatography

In this study, we investigated low pH inactivation of viruses including PCV Type 1 (PCV1) and PCV1 removal by cation exchange chromatography (CEX) in the presence of pepsin. Both parvovirus and PCV1 could be effectively inactivated by low pH and PCV1 could be removed by POROS 50HS CEX. The POROS 50HS method presented in this article is helpful for designing other CEX methods for the same purpose and not much difference would be expected for similar product intermediates and same process parameters. While the effectiveness needs to be confirmed for specific applications, the results demonstrate that both low pH (pH 1.7) and CEX methods were successful in eliminating PCV1 and thus either can be considered as an effective virus barrier.

Source: Biotechnology Progress - February 14, 2020 Category: Biotechnology Authors: Bin Yang, Hua Wang, Kimberly Kaleas, Michelle Butler, Jayme Franklin, Amber Bill, Sally A. Baylis, Qi Chen, Johannes Bl ümel Tags: Research Article Source Type: research