Heat shock protein 60 is a disease-associated sialoglycoprotein in human non-small cell lung cancer.

Heat shock protein 60 is a disease-associated sialoglycoprotein in human non-small cell lung cancer. Biol Chem. 2019 Dec 01;: Authors: Singh P, Kumari M, Bal A, Srinivasan R, Ghosh S Abstract The diagnostic and therapeutic potential of Maackia amurensis agglutinin (MAA) have been reported in various malignancies. Earlier, we have found that MAA specifically interacted with human non-small cell lung-cancer (NSCLC) cells and induced apoptosis in these cells. The present study was designed to identify Maackia amurensis leukoagglutinin (MAL-I, one of the components of MAA, having same carbohydrate specificity as MAA) interacting membrane sialoglycoprotein(s) of two subtypes of human NSCLC cell lines. Nine proteins were identified using 2D-PAGE followed by MAL-I-overlay transblotting and MALDI-TOF mass spectrometry. Among these proteins, HSP60 was selected for further characterization. Sialoglycoprotein nature of membrane-HSP60 of NSCLC cell lines was confirmed by its reduced reactivity with MAL-I in Western blots in presence of GM2 and by dual staining of the cell lines with MAL-I and HSP60-antibody. These findings were further substantiated by enzymatic analysis of membrane-HSP60 as well as in-silico evidence regarding this protein. Our observations were validated by immunohistochemical analysis of both subtypes of NSCLC tissue sections. Membrane-HSP60 was found to be involved in inhibition of MAL-I induced morphological alteration of N...
Source: Biological Chemistry - Category: Chemistry Tags: Biol Chem Source Type: research