Development of a multiplex real-time PCR assay for rapid detection of tigecycline resistance gene tet(X) variants from bacteria, faeces, and environment samples.

Development of a multiplex real-time PCR assay for rapid detection of tigecycline resistance gene tet(X) variants from bacteria, faeces, and environment samples.
Antimicrob Agents Chemother. 2020 Feb 10;:
Authors: Fu Y, Liu D, Song H, Liu Z, Jiang H, Wang Y
Abstract
We developed a multiplex real-time SYBR Green-based PCR assay for rapid detection of tet(X) and its variants, including tet(X1), tet(X2), and high-level tigecycline resistance genes tet(X3), tet(X4) and tet(X5). High linearity (R2 ≥0.996), sensitivity (low detection limit) and specificity (only the target gene could be amplified significantly) were showed in developed real-time PCR assay and further evaluated using bacterial, faeces, and environmental samples.
PMID: 32041710 [PubMed - as supplied by publisher]

Source: Antimicrobial Agents and Chemotherapy - February 9, 2020 Category: Microbiology Authors: Fu Y, Liu D, Song H, Liu Z, Jiang H, Wang Y Tags: Antimicrob Agents Chemother Source Type: research