Datura suaveolens and Verbena tenuisecta mediated silver nanoparticles, their photodynamic cytotoxic and antimicrobial evaluation

AbstractBiogenic production of nanoparticles is eco-friendly, less expensive method with various medical and biological applications. Nanotechnology along with photodynamic therapy is gaining tremendous importance with enhanced efficacy. The present work was aimed to evaluate methanolic extracts and nanoparticles of two selected plants (Datura suavolens andVerbina tenuisecta) for cytotoxic photodynamic, antioxidant and antimicrobial study. Both extract and silver (5  mM) nanoparticles ofDatura plant showed significant activities against bacterial strains. Maximum ZOI of 27.3  ± 1.6 mm was observed with nanoparticles ofDatura branches with minimum inhibitory (MIC) value of 32  µg/ml. In case of antifungal and antioxidant assay samples were moderately active. Silver nanoparticles and extracts were effective against rhabdomyosarcoma cell line with lowest IC50 value of 42.5  ± 0.6 μg/ml and percent viability of 25.6 ± 1.3 ofVerbena tenuisecta. However, nanoparticles ofDatura leaves and branches were more potent with IC50 value of 2.4  ± 0.9 μg/ml and 7.8 ± 1.1 μg/ml respectively. The result of photodynamic study showed that efficacy of photosensitizer was enhanced and percent viability reduced when nanoparticles used as an adjunct. The color change and UV spectra (415‒425 nm) indicated the production of nanopart icles. Fourier transform infrared spectroscopy (FTIR) spectra showed presence of different functional groups e.g., hydroxyl, carbony...

Source: World Journal of Microbiology and Biotechnology - February 10, 2020 Category: Microbiology Source Type: research