Structural characterization, antioxidant and immunomodulatory activities of a neutral polysaccharide from Cordyceps militaris cultivated on hull-less barley

Publication date: Available online 10 February 2020Source: Carbohydrate PolymersAuthor(s): Ying Zhang, Yan Zeng, Yongsheng Cui, Hanmin Liu, Caixia Dong, Yuanxia SunAbstractA water-soluble neutral polysaccharide (SDQCP-1) was isolated from fruit bodies of Cordyceps militaris cultivated on hull-less barley. SDQCP-1 was composed of mannose, glucose and galactose in the mole ratio of 13.3:1.0:9.7, with an average molecular weight of 19.3 kDa. Based on results from methylation analysis, GC-MS and NMR, SDQCP-1 was elucidated to be a glucogalactomannan with a backbone composed of (1→2)-α-D-Manp (48.4%) and (1→4)-β-D-Glcp (1.2%) residues. Its side chains were branched at O-6 position of (1→2)-α-D-Manp mainly by (1→2)-β-D-Galf or (1→6)-α-D-Manp residues which were terminated mainly with α-D-Galf, α-D-Galp residues. Besides exhibiting a good antioxidant capacity with an ORACFL value of 24.7 mmol Trolox/g and a TEAC value of 202.4 μmol Trolox/g, SDQCP-1 also could stimulate macrophages to release NO, TNF-α, IL-6, and IL-10, and mainly induced M1 polarization of macrophages. The findings indicated that SDQCP-1, from C. militaris cultivated on hull-less barley, could be used as potential natural antioxidant and immunomodulator in functional foods or medicine.
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research