An automated workflow approach for the analysis of flavin adenine dinucleotide by HPLC with internal standard.

In this study we have developed a fully automated method for the analysis of FAD by fluorescence detection. FAD is extracted from whole blood samples by trichloroacetic acid, with diethyl ribityl isoalloxazine used as an internal standard. Linearity for FAD was above 0.99 (r2) up to a concentration range of 1000 nmol/L. Precision of the method (intra-day and inter-day) compared against commercial quality control material was below 8% (coefficient of variation) with recovery of FAD exceeding 90%. Accuracy of the protocol was compared against a previous cycle from an external quality assurance program (RCPAQAP) (n = 12) with satisfactory agreement. Overall, this method has increased laboratory workflow and reduced manual labour required in performing FAD analysis. PMID: 32035844 [PubMed - as supplied by publisher]

https://www.ncbi.nlm.nih.gov/pubmed/32035844?dopt=Abstract

Source: Analytical Biochemistry - February 5, 2020 Category: Biochemistry Authors: Nguyen VL, Ah-Loo A, Fitzpatrick M Tags: Anal Biochem Source Type: research