Controllable accumulation of conjugated polymer nanoparticles on the surface of adhesive bacteria

Publication date: Available online 9 February 2020Source: Colloids and Surfaces A: Physicochemical and Engineering AspectsAuthor(s): Lu Liu, Xiaoyu Wang, Dongdong Ban, Shuxian Zhu, Lidong LiAbstractBacterial adhesions have caused serious infections and gradually evolved into a health threat, especially with the development of drug resistance in recent years. In this work, we report about controlling accumulation of conjugated polymer nanoparticles (CPNs) on the surface of adhesive bacteria, achieving anti-adhesion and sterilization simultaneously. Firstly, we prepared PFPPBA CPNs that had phenylboronic acid (PBA) and quaternary ammonium groups on surface. Then mannotriose was introduced to the bacterial adhesion system. Based on the FimH/mannose recognition, mannotriose occupied adhesion sites of adhesive E. coli and inhibited bacterial adhesion to cells. Importantly, the mannotriose on bacterial surface promoted CPNs actively accumulated on bacteria due to the high affinity between diols of mannotriose and PBA of CPNs. Finally, the bacteria were killed by dark toxicity and ROS generated by CPNs without producing drug-resistance. Hence, through using mannotriose, we developed a facile approach to control accumulation of CPNs on bacterial surface and inhibit bacterial adhesion to host cells. This strategy provides a novel insight into antiadhesive treatment of bacteria and sterilization applications.Graphical abstract

Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects - February 10, 2020 Category: Chemistry Source Type: research