Validation of triple-wash procedure with a H2O2-peroxyacetic acid mixer to improve microbial safety and quality of butternut squashes and economic feasibility analysis

This study aims to i) evaluate two triple-wash procedures with an H2O2-peroxyacetic-acid mixer (SaniDate-5.0) to improve microbial safety and quality of butternut squashes and ii) determine the feasibility of the triple-wash application in a processing plant. In study I, fresh squashes were dip-inoculated with a mixture of Salmonella and Listeria monocytogenes, followed by two triple-wash steps including water dip-antimicrobial dip-water dip (WAW) or water dip-water dip-antimicrobial dip (WWA). Tested antimicrobials were i) lactic/citric acid blend (LCA; 2.5%); ii) sodium hypochlorite (SH; 100 ppm); and iii) SaniDate-5.0, 0.0064, 0.25 and 0.50%. Surviving bacteria were recovered using XLT-4 agar for Salmonella and MOX agar for L. monocytogenes. In study II, freshly harvested squashes were either left unwashed or triple-washed using WWA in water and SaniDate-5.0 (0.0071 and 0.45%) at a processing plant followed by storage at 9 °C for 70 days. Aerobic Plate Counts (APCs), coliforms/Escherichia coli, lactic-acid-bacteria, and psychrotrophs on squashes were tested every seven days. Counts of Salmonella and L. monocytogenes on unwashed squash were 5.0–5.3 and 5.4–6.0 log CFU/squash, respectively. WWA wash was more effective (P 
Source: Food Control - Category: Food Science Source Type: research

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Publication date: Available online 15 March 2020Source: Food ControlAuthor(s): Lina Sheng, Zheng Zhang, Gang Sun, Luxin Wang
Source: Food Control - Category: Food Science Source Type: research
Conclusion: The actual results indicate that the high levels of bacterial counts on kitchen surfaces, presents an evident need to improve the hygienic process and adopt an HACCP system in this facility. PMID: 32148685 [PubMed]
Source: Iranian Journal of Microbiology - Category: Microbiology Tags: Iran J Microbiol Source Type: research
This study was conducted to address the dearth in works that simultaneously compare the growth and inactivation behaviors of selected pathogens in different milk products. In worst-case scenarios where hygienic practices are absent and heavy microbiological contaminations occur, Salmonella enterica, Escherichia coli O157:H7, Listeria monocytogenes, Pseudomonas aeruginosa, and Staphylococcus aureus multiplied in all samples at room temperature (27 °C). Most organisms readily proliferated with growth lag (tlag) values ranging from 0.00 to 5.95 h. Growth rates (KG) ranged from 0.16 to 0.67 log CFU/h. Sanitary ri...
Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research
This study was aimed at assessing whether the repeated exposure of twelve strains of Salmonella spp., Escherichia coli and Listeria monocytogenes to the alternative non-thermal decontamination techniques ultraviolet light (UV-C) and non-thermal atmospheric plasma (NTAP) may cause the emergence of variants showing an increased resistance to clinically relevant antibiotics (ampicillin, cefotaxime, ciprofloxacin, gentamicin, streptomycin, tetracycline, erythromycin, vancomycin and colistin). UV-C and NTAP treatments were applied on the surface of inoculated BHI agar plates. Survivors were recovered and, after 24 hours growth ...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
Abstract The work synthesized the fungal chitosan (FCS) encapsulated Gynura procumbens (GP) mediated silver nanoparticles (GP-AgNPs) for enhanced antidiabetic, antioxidant and antibacterial activity. The FCS-GP-AgNPs were characterized through UV-Visible spectroscopy, FTIR, XRD, Zeta size analyzer and TEM. The FTIR spectrum of GP-AgNPs exhibited functional groups of phenolic and flavonoids. The crystal peaks related to silver and chitosan in FCS-GP-AgNPs were demonstrated by XRD spectrum. The polydispersed nanoparticles such as AgNPs and FCS-GP-AgNPs were observed with size
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research
Publication date: Available online 19 February 2020Source: Materials Chemistry and PhysicsAuthor(s): Natasha Vieira Sievers, Liliane Damaris Pollo, Gertrudes Corção, Nilo Sérgio Medeiros CardozoAbstractThis work reports a new method to obtain polypropylene/titanium dioxide (PP/TiO2) films with antibacterial activity through the in situ synthesis of nanostructured TiO2 directly in the polymer solution. In order to compare the performance of the different types of particles, TiO2 particles were also prepared utilizing the sol-gel method and subsequently incorporated into the polymer to form PP/TiO2 films...
Source: Materials Chemistry and Physics - Category: Materials Science Source Type: research
In this study we designed and synthetized various derivatives of NCR247, including shorter fragments and various chimeric derivatives. The antimicrobial activity of these peptides was tested on the ESKAPE bacteria; Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Escherichia coli as a member of Enterobacteriaceae and in addition Listeria monocytogenes and Salmonella enterica. The 12 amino acid long C-terminal half of NCR247, NCR247C partially retained the antimicrobial activity and preserved the multitarget interactions with partners of NCR247. Nevert...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In conclusion, unlike traditional alheiras which often contain pathogenic agents, no harmful organisms nor chemical hazards were found in these new products, even though produced by the same companies.
Source: Food Control - Category: Food Science Source Type: research
This study characterized Listeria monocytogenes, Salmonella enterica, and E. coli O157:H7 by collecting rectoanal mucosal swabs and fecal samples from 518 imported beef cattle at Jordan’s major abattoir. A unique 53 L. monocytogenes, 287 S. enterica, and 17 E. coli O157:H7 were isolated from 37, 120 and 9 different animals; respectively. The prevalence of S. enterica, L. monocytogenes and E. coli O157:H7 were 23.2% (95% CI, 19.7-27.0%), 7.1% (95% CI, 5.2-9.7%) and 1.7% (95% CI, 0.9-3.3%); respectively. All L. monocytogenes, all E. coli O157:H7 and 93.0% of S. enterica isolates resisted at least one antimicrobial clas...
Source: Comparative Immunology, Microbiology and Infectious Diseases - Category: Infectious Diseases Source Type: research
This study characterized Listeria monocytogenes, Salmonella enterica, and E. coli O157:H7 by collecting rectoanal mucosal swabs and fecal samples from 518 imported beef cattle at Jordan's major abattoir. A unique 53 L. monocytogenes, 287 S. enterica, and 17 E. coli O157:H7 were isolated from 37, 120 and 9 different animals; respectively. The prevalence of S. enterica, L. monocytogenes and E. coli O157:H7 were 23.2 % (95 % CI, 19.7-27.0 %), 7.1 % (95 % CI, 5.2-9.7 %) and 1.7 % (95 % CI, 0.9-3.3 %); respectively. All L. monocytogenes, all E. coli O157:H7 and 93.0 % of S. enterica isolates resisted at least one antimicrobial ...
Source: Comparative immunology, microbiology and infectious diseases. - Category: Allergy & Immunology Authors: Tags: Comp Immunol Microbiol Infect Dis Source Type: research
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