Equilibrium data and thermodynamic studies of L-tryptophan partition in alcohol / phosphate potassium salt-based aqueous two phase systems

Publication date: Available online 25 January 2020Source: The Journal of Chemical ThermodynamicsAuthor(s): Seyyed Mohammad Arzideh, Kamyar Movagharnejad, Mohsen PirdashtiAbstractExperimental phase diagrams for {1-butanol/2-butanol/t-butyl alcohol+ potassium hydrogen phosphate (K2HPO4) + water} at 298.15 K have been determined, and their ability to separate an amino acid, L-Tryptophan, were measured. An excess Gibbs energy model has been proposed for modelling of aqueous two-phase systems (ATPS) containing light straight/branched alcohol, K2HPO4, and water. The hybrid ion-interaction and solvation model (HIS) was implemented to calculate the long-range ion-ion and middle-range ion-solvent interactions, and the UNIQUAC model was used for the short-range solvent-solvent interactions. The continuum characteristics such as density, dielectric constant, and solvation parameters were considered as mixed-solvent property dependent correlations. The proposed excess Gibbs energy model, HIS-UNIQUAC, has been found to describe the LLE data in a satisfactory precision of less than 0.331% for mass percent of experimental data. Moreover, the partitioning coefficient shows a perfect correlation (R2>0.982) with the long and middle range term of excess Gibbs energy model.Graphical abstract
Source: The Journal of Chemical Thermodynamics - Category: Chemistry Source Type: research