Inhibition of Wnt signalling pathway by XAV939 enhances radiosensitivity in human cervical cancer HeLa cells.

Inhibition of Wnt signalling pathway by XAV939 enhances radiosensitivity in human cervical cancer HeLa cells. Artif Cells Nanomed Biotechnol. 2020 Dec;48(1):479-487 Authors: Zhang J, Si J, Gan L, Guo M, Yan J, Chen Y, Wang F, Xie Y, Wang Y, Zhang H Abstract Cervical cancer is the second most common malignant tumour threatening women's health. In recent years, heavy-ion beam therapy is becoming a newly emerging therapeutic mean of cancer; however, radio-resistance and radiation-induced damage constitute the main obstacles for curative treatment of cervical cancer. Therefore, to identify the radiosensitizers is essential. Here, we investigated the effects of Wnt signalling pathway on the response of 12C6+ radiation in HeLa cells. XAV939, an inhibitor of Wnt signalling pathway, was added two hours before 12C6+ radiation.12C6+ radiation inhibited the viability of HeLa cells in a time-dependent manner, and inhibiting Wnt signalling using XAV939 significantly intensified this stress. Meanwhile, 12C6+ radiation induced a significant increased cell apoptosis, G2/M phase arrest, and the number of γ-H2AX foci. Supplementation with XAV939 significantly increased the effects induced by 12C6+ radiation alone. Combining XAV939 with 12C6+ irradiation, the expression of apoptotic genes (p53, Bax, Bcl-2) was significantly increased, while the expression of Wnt-related genes (Wnt3a, Wnt5a, β-catenin, cyclin D1 and c-Myc) was significantly decreased. Overall, the...
Source: Artificial Cells, Nanomedicine and Biotechnology - Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

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In this study, the cytotoxicity of ZnO QDs was assessed using HeLa cervical cancer cell and HEK-293T human embryonic kidney cell lines. Cell viability was significantly decreased by treatment with 50 µg/ml ZnO QDs after only 6 h, and the cytotoxicity of ZnO QDs was higher in HEK-293T than in HeLa cells. ZnO QDs increased the level of reactive oxygen species and decreased the mitochondria membrane potential in a dose-dependent manner. Several gene expression involved in apoptosis was regulated by ZnO QDs, including bcl-2 gene and caspase. In HeLa cells, ZnO QDs significantly increased early and late apoptosis, but onl...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
CONCLUSION: Ultrasound and MRI have similar diagnostic performance for detecting parametrial infiltration in women with cervical cancer. This might have relevance from the clinical point of view, since ultrasound is cheaper than MRI. PMID: 32096793 [PubMed - as supplied by publisher]
Source: Medical Ultrasonography - Category: Radiology Tags: Med Ultrason Source Type: research
Condition:   Cervical Cancer Interventions:   Other: Community-based HPV screening strategy;   Other: Clinic-based HPV screening strategy Sponsors:   University of North Carolina, Chapel Hill;   United States Agency for International Development (USAID) Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Authors: Miao H, Song WB, Zhu H, Wang Q, Tian Y Abstract OBJECTIVE: To investigate the effect of GREM1 on the sensitivity of cervical squamous carcinoma cells to radiotherapy and chemotherapy. PATIENTS AND METHODS: The resected cancer tissues and paracancerous tissues of patients with cervical carcinoma were collected. The expressions of GREM1 protein and mRNA in SiHa cell line of cervical squamous carcinoma were tested by Western blot and reverse transcription-polymerase chain reaction (qRT-PCR). The effect of GREM1 on chemotherapy sensitivity of SiHa cells was tested by MTT assay. SiHa cells were irradiated w...
Source: European Review for Medical and Pharmacological Sciences - Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
[New Era] Keetmanshoop -Cervical cancer is on the rise in Namibia and this has made the government to regard the disease a public health concern, said //Kharas Regional Council chairperson, Jan Scholtz.
Source: AllAfrica News: Health and Medicine - Category: African Health Source Type: news
[Daily News] CONCERTED efforts are needed to ensure that no woman dies from cervical cancer, the leading cause of cancer and cancer-related deaths among women.
Source: AllAfrica News: Health and Medicine - Category: African Health Source Type: news
[FrontPageAfrica] Monrovia -Cervical Cancer accounts for more than 40 percent of all women suffering from cancer in the world and about two-thirds of cancer deaths. In Liberia, it is the most common cancer affecting women aged 15 - 44, says Joyce Killikpo, Executive Director of Public Health Initiative of Liberia, PHIL - a local non-for-profit organization working to curb the medical condition that remains a serious menace for women across the world.
Source: AllAfrica News: Health and Medicine - Category: African Health Source Type: news
CONCLUSIONS The expression of MyD88 and TRAF6 might vary in different stages of HPV infection. pDCs might regulate CD40 to participate in the tumorigenesis and progression of cervical cancer, but related mechanisms still need further investigation. PMID: 32089541 [PubMed - in process]
Source: Medical Science Monitor - Category: Research Tags: Med Sci Monit Source Type: research
Publication date: Available online 24 February 2020Source: The Lancet Global HealthAuthor(s): Kaja M Abbas, Kevin van Zandvoort, Marc Brisson, Mark Jit
Source: The Lancet Global Health - Category: International Medicine & Public Health Source Type: research
Conclusions: Although in many cases CIN tends to regress spontaneously after delivery, such outcome is not to be expected in all patients. When invasive cervical cancer has been excluded, definitive treatment for cervical dysplasia may be deferred to the postpartum period without any harm to the mother.
Source: Indian Journal of Cancer - Category: Cancer & Oncology Authors: Source Type: research
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