Generation of induced pluripotent stem cell line (ZZUi0016-A) from dermal fibroblasts of a normal human

Publication date: Available online 25 January 2020Source: Stem Cell ResearchAuthor(s): Yuan Gao, Yanlin Wang, Zhuoya Wang, Huifang Sun, Rui Zhang, Jing Yang, Yutao Liu, Han Liu, Qi Zhang, Shoutao Zhang, Changhe Shi, Yuming Xu, Bo SongAbstractDermal fibroblasts obtained from a 43-year-old healthy man were successfully transformed into induced pluripotent stem cells (iPSCs) by employing episomal plasmids expressing OCT3/4, SOX2, KLF4, LIN28, and L-MYC. The iPSCs showed a normal karyotype and exhibited the potential to differentiate into three germ layers in a teratoma assay, which is often used to assess the pluripotency of stem cells. This iPSC line may be subsequently used for drug screens, biological tissue engineering, and cell transplantations.
Source: Stem Cell Research - Category: Stem Cells Source Type: research