Adenomyosis-derived extracellular vesicles endow endometrial epithelial cells with an invasive phenotype through epithelial-mesenchymal transition

In this study, AMEVs were isolated from patients with adenomyosis and characterized by transmission electron microscopy, Western blot, and nanoparticle tracking. Primary endometrial epithelial cells (EECs) were derived from normal endometrium tissues from patients with leiomyoma and co-cultured with AMEVs in vitro. AMEV uptake was examined by fluorescence confocal microscopy. The invasion of EECs was confirmed by Transwell assay. Immunohistochemistry, Western blot, and qRT-PCR were performed on EECs to illustrate the expression levels of cytokeratin 19, E-cadherin, vimentin, and zinc finger E-box-binding homeobox 1 (ZEB1). The results indicated that the cellular fluorescence intensity gradually increased after 48 hours of co-culture, but decreased after 72 hours. After co-culturing with AMEVs for 72 hours, EECs expressed significantly lower levels of cytokeratin 19 and E-cadherin, and significantly higher levels of vimentin and ZEB1. Together these results demonstrated that AMEVs induce an EMT process and enhance the invasion of EECs. These changes may contribute to the pathogenesis and progression of adenomyosis.
Source: Genes and Diseases - Category: Genetics & Stem Cells Source Type: research

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Authors: Samanci C, Önal Y Abstract BACKGROUND/AIM: We aimed to investigate the role of Shearwave Elastography (SWE) in the evaluation of response to uterine artery embolization (UAE) in patients with uterine leiomyomas. MATERIALS AND METHODS: SWE images of the dominant uterin leiomyomas were obtained before and 1.5 months after performing UAE in 33 women suffering from symptoms due to leiomyomas (menometrorrhagia, bulk-related symptoms, pelvic pain). Leiomyomas were also evaluated for location and longest diameter in axial plane by two observers. Inter observer agreement in the quantitative SWE analysis w...
Source: Turkish Journal of Medical Sciences - Category: General Medicine Tags: Turk J Med Sci Source Type: research
Conditions:   Uterine Leiomyoma;   Surgical Procedure, Unspecified Interventions:   Procedure: RM;   Procedure: LM Sponsors:   Taipei Medical University Shuang Ho Hospital;   Tri-Service General Hospital;   Taipei Medical University WanFang Hospital;   Asian Society for Gynecologic Robotic Surgery (ASGRS) Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
A 39-year-old nulligravid woman underwent laparoscopic myomectomy for a 6cm posterior fundal leiomyoma (FIGO Type 2-5). The uterine serosa was incised with monopolar electrosurgery and the myoma was enucleated using a laparoscopic corkscrew with blunt dissection. During this process, the endometrial cavity was entered. The endometrium was reapproximated with absorbable suture in a running fashion. The overlying myometrium was closed in three layers using barbed delayed absorbable suture in a running fashion.
Source: The Journal of Minimally Invasive Gynecology - Category: OBGYN Authors: Tags: IMAGES IN Gynecological Surgery Source Type: research
Authors: Abstract Chronic pelvic pain is a common, burdensome, and costly condition that disproportionately affects women. Diagnosis and initial management of chronic pelvic pain in women are within the scope of practice of specialists in obstetrics and gynecology. The challenging complexity of chronic pelvic pain care can be addressed by increased visit time using appropriate coding modifiers, as well as identification of multidisciplinary team members within the practice or by facilitated referral. This Practice Bulletin addresses the diagnosis and management of chronic pelvic pain that is not completely explai...
Source: Obstetrics and Gynecology - Category: OBGYN Tags: Obstet Gynecol Source Type: research
Authors: Abstract Chronic pelvic pain is a common, burdensome, and costly condition that disproportionately affects women. Diagnosis and initial management of chronic pelvic pain in women are within the scope of practice of specialists in obstetrics and gynecology. The challenging complexity of chronic pelvic pain care can be addressed by increased visit time using appropriate coding modifiers, as well as identification of multidisciplinary team members within the practice or by facilitated referral. This Practice Bulletin addresses the diagnosis and management of chronic pelvic pain that is not completely explai...
Source: Obstetrics and Gynecology - Category: OBGYN Tags: Obstet Gynecol Source Type: research
Conclusion: Serum levels of cox2 and wnt3a exhibited diagnostic value for endometrial cancer. Cox2 serum levels and β-catenin expression also showed potential value of prognostic prediction. Cox2 serum levels might be a potential marker for early diagnosis and prognosis prediction in endometrial cancer.
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
Abstract“Benign” metastatic leiomyomas (BML) are indolently growing metastatic tumors which mostly associate with uterine leiomyomas in women in reproductive ages. The reason to define these lesions as “benign” despite metastasis is their pathological features with low mitotic counts, lack of or min imal nuclear atypia, pseudocyst formation, and coagulative necrosis unlike leiomyosarcomas. Despite lack of pathological malignant features, they may cause significant morbidity and even mortality. Here, we describe a BML case with metastases to vertebrae and skull bones. Vertebral and skull metasta ses ...
Source: Neurosurgical Review - Category: Neurosurgery Source Type: research
Conclusions: Clear exposure of gastric SETs during MCB may improve the diagnostic rate of such examinations. PMID: 32053861 [PubMed - as supplied by publisher]
Source: Clinical Endoscopy - Category: Gastroenterology Tags: Clin Endosc Source Type: research
First step in evaluating a woman with clinical suspicion of leiomyomas is to look into typical symptoms such as heavy or prolonged menstrual bleeding, bulk related symptoms (pelvic pressure or pain, constipation, and dyspareunia) or reproductive dysfunction. Fibroids are sometimes found in asymptomatic women during routine pelvic examination or incidentally during imaging. The clinician should also perform a precise physical examination comprising of abdominal and pelvic examination. The abdominal examination should include palpation for a pelvic or abdominal mass and the uterine fundus should be noted.
Source: European Journal of Obstetrics, Gynecology, and Reproductive Biology - Category: OBGYN Authors: Tags: Full length article Source Type: research
Uterine leiomyomas are the most common benign tumors of the uterus with a premenopausal cumulative clinically relevant incidence of 50% in black and 35% in white women [1]. The standard treatment for symptomatic fibroids is hysterectomy, but there has been a shift towards less invasive approaches like myomectomy, high intensity focused ultrasound and UFE [1 –3]. Mid- and long-term studies evaluating therapy effectiveness after UFE suggest freedom from treatment failure between 70%-90%, based on follow-up times between 1 and 10 years [2,3,5–9]; however, follow-up data longer than 10 years after UFE...
Source: European Journal of Obstetrics, Gynecology, and Reproductive Biology - Category: OBGYN Authors: Tags: Review article Source Type: research
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