Optimized immobilization of endoglucanase Cel9A onto glutaraldehyde activated chitosan nanoparticles by response surface methodology: The study of kinetic behaviors

In this study, chitosan nanoparticles (ChNPs) were prepared by Ionic gelation method and Endoglucanase Cel9A fromAlicyclobacillus acidocaldariius (AaCel9A) immobilized on the nanoparticles. The FTIR results showed that the enzymes were immobilized on the ChNPs. The dynamic light scattering and scanning electron microscope (SEM) results illustrated that the AaCel9A ‐ChNPs approximately had 40 nm diameters. For optimizing enzyme immobilization, response surface methodology was employed using different variables (pH, enzyme immobilization time, and enzyme to ChNPs ratio [E/Cs]). The results showed that the high immobilization efficiency was achieved in pH 7, E/Cs of 0.4 in 2.63 hr. The enzyme activity results showed that, immobilization increased optimum pH for activity (from 6.5 to 7.5) and the enzymeKm (from 3.703 to 12.195 [mg/ml]), which make it suitable to use in some industries such as detergents.

Source: Biotechnology Progress - January 20, 2020 Category: Biotechnology Authors: Saeed Najavand, Mahdiyeh Habibnejad, A. R. Amani ‐Ghadim, Parastou Rahimizadeh, Mohammad Pazhang Tags: RESEARCH ARTICLE Source Type: research