Effect of traditionally used herb Pedalium murex L. and its active compound pedalitin on urease expression – for the management of kidney stone

Publication date: Available online 16 January 2020Source: Saudi Journal of Biological SciencesAuthor(s): S. Ramadevi, B. Kaleeswaran, S. Ilavenil, Akilesh upgade, D. Tamilvendan, R. Rajakrishnan, A.H. Alfarhan, Y.-O. Kim, H.-J. KimAbstractPedalium murex L. is a medicinal herb that has been used for the treatment of diseases related to kidney in the traditional system of medicine. The current study aims to study the effect of ethyl acetate extract of P. murex (EAEP) and its fractionated compound pedalitin against urease production and UreC gene expression in Proteus mirabilis.The selected reference strain Proteus mirabilis (MTCC 425) and the isolates culture of Proteus mirabilis were subjected to study the antibacterial efficacy of P. murex. Expression analysis ofP. mirabilis urease gene was successfully done by QPCR. The ethyl acetate extract effectively inhibit the reference Proteus mirabilis and bacterial isolates of Proteus mirabilis in hospitalized urine sample.EAEP has showed more potent activity (56.7%) against urease enzyme and pedalitin also has potent activity value (30.1%). Using qPCR, the expression of UreC gene of P. mirabilis was controlled by EAEP and also its bioactive compound pedalitin. The present study clearly demonstrated the potency of P. murex in controlling the growth of pathogenic P. mirabilis and to control the expression of urease enzyme production as well as to restrict the urease gene expression in P. mirabilis.
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research

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