Discrimination of Moutan Cortex from different sources and geographical origins for quality evaluation using microsatellite markers coupled with chemical analysis
Publication date: April 2020Source: Biochemical Systematics and Ecology, Volume 89Author(s): Yingchun Zhang, Mei Ge, Xinhui Wang, Yue Zeng, Yixuan Liao, Yapeng Wang, Zhongliang ZengAbstractThe root bark of tree peony (Paeonia suffruticosa Andr.), also called Coetex Moutan (CM), is a traditional Chinese medicine. The medicinal plant is also well known for its beautiful flowers with hundreds of cultivars. CM from different cultivars or grown in various geographic areas are significantly different in quality and chemical properties. So the aim of this study is to develop specific SSRs by transcriptome analysis to distinguish CM samples from different sources and quantify the contents of paeonol and paeoniflorin to evaluate its chemical qualities. Totally, 6654 SSR markers were developed in the coding regions by transcriptome. 10 pairs of SSRs were found to be consistently polymorphic when applied in all fifty individuals collected from three typical producing areas (Chongqing, Henan, and Anhui province). These CM individuals were originated from two medicinal cultivars of P. suffruticosa and Paeonia ostii T. Hong et J. X. Zhang and horticultural varieties. The genetic results indicated that fifty samples could be clearly divided into three clusters based on genetic distance, in which samples from the same species all gathered together without being affected by geographical origins. The accumulations of two main chemical markers showed great variation among tree peony species or ...
Publication date: Available online 3 June 2020Source: The Journal of Molecular DiagnosticsAuthor(s): Anthony N. Sireci, Jay L. Patel, Loren Joseph, Matthew C. Hiemenz, Oana C. Rosca, Samuel K. Caughron, Sarah A. Thibault-Sennett, Tara L. Burke, Dara L. Aisner
Publication date: Available online 2 June 2020Source: Pathology - Research and PracticeAuthor(s): Theodore Vougiouklakis, Brendan J. Belovarac, Andrew Lytle, Luis Chiriboga, Ugur Ozerdem
Publication date: Available online 2 June 2020Source: Pathology - Research and PracticeAuthor(s): Hyeongjoo Kim, Taewan Kim, Gunn Jaygal, Jongsoo Woo, Chang-Jin Kim, Moo-Jun Baek, Dongjun Jeong
Publication date: Available online 2 June 2020Source: Pathology - Research and PracticeAuthor(s): Qian Wu, Weiya Wang, Ping Zhou, Yiyun Fu, Ying Zhang, Yang W. Shao, Lili Jiang
Publication date: Available online 2 June 2020Source: The Journal of Foot and Ankle SurgeryAuthor(s): Sergio Agudiez Calvo, Jorge Ballesteros de Frutos, Héctor Raúl Cabezas García, Daniel Pecos Martin, Tomás Gallego Izquierdo
Publication date: Available online 2 June 2020Source: Orthopaedics &Traumatology: Surgery &ResearchAuthor(s): Alexandre Hardy, Guillaume Lefebvre
Publication date: Available online 2 June 2020Source: Journal of Orthopaedic TranslationAuthor(s): Zihao He, Linyang Chu, Xuqiang Liu, Xuequan Han, Kai Zhang, Mengning Yan, Xiaofeng Li, Zhifeng Yu
Publication date: Available online 3 June 2020Source: Journal of Clinical Orthopaedics and TraumaAuthor(s): Alaa A. Dawood
This study aims to assess whether older adults with low muscle mass or strength, in the presence of obesity, have an increased risk of knee (TKR) and hip replacement (THR) over 13 years. 1082 community-dwelling older adults (51% women; mean age 62.9 ± 7.5 years) were studied at baseline and multiple time points over 13 years. The incidence of TKR and THR was determined by data linkage to National Joint Replacement Registry. Appendicular lean and fat mass were measure d using DXA. Lower-limb muscle strength (LMS) was assessed by dynamometer. Low muscle mass and strength were defined as t...
Publication date: Available online 2 June 2020Source: Journal of OptometryAuthor(s): Tahereh Rakhshandadi, Mohamad-Reza Sedaghat, Farshad Askarizadeh, Hamed Momeni-Moghaddam, Mehdi Khabazkhoob, Abbasali Yekta, Foroozan Narooie-Noori