Betaine supplementation mitigates intestinal barrier disintegrity and bacterial endotoxin translocation in cirrhotic rats

Publication date: Available online 16 January 2020Source: PharmaNutritionAuthor(s): Mohammad Mehdi Ommati, Omid Farshad, Khadijeh Mousavi, Akram Jamshidzadeh, Mohammadreza Azmoon, Sirus Heidari, Negar Azarpira, Hossein Niknahad, Reza HeidariAbstractGut barrier disintegrity and high serum bacterial endotoxin (Lipopolysaccharide; LPS) level is a prominent clinical challenge in cirrhotic patients. Several investigations mentioned the pivotal role of oxidative stress in this complication. Betaine (BET) is widely investigated for its physio/pharmacological properties. It has been found that BET mitigates oxidative stress in different experimental models. In the current study, the effects of BET supplementation (0.5 and 1% w: v, gavage) on biomarkers of oxidative stress, tissue histopathological alterations, and LPS translocation in cirrhotic rats was evaluated. Rats underwent bile duct ligation (BDL) and supplemented with BET for 28 consecutive days. Significant increase in ileum and colon reactive oxygen species (ROS), lipid peroxidation, oxidized glutathione (GSSG) levels, and protein carbonylation along with decreased antioxidant capacity and reduced glutathione (GSH) content were evident in BDL rats. Mitochondrial indices, including mitochondrial permeabilization, ATP content, and mitochondrial membrane potential were also significantly different in the intestine of BDL animals. Tissue histopathological alterations, as well as high serum LPS, were also detected in cirrhotic an...
Source: PharmaNutrition - Category: Nutrition Source Type: research

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Publication date: 25 February 2020Source: Cell Reports, Volume 30, Issue 8Author(s): Hema Chandra Kotamarthi, Robert T. Sauer, Tania A. Baker
Source: Cell Reports - Category: Cytology Source Type: research
Conclusion: The study showed that TET1 dysfunction inhibited the migration and invasion of BCPAP cells and might have a potential role in the pathogenesis of PTC. PMID: 32089682 [PubMed]
Source: International Journal of Endocrinology - Category: Endocrinology Tags: Int J Endocrinol Source Type: research
Contributors : Li R Kong ; Tuan Z TanSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensMutations in the DNA binding domain (DBD) of TP53 are often associated with the gain-of-function (GOF) of mutp53, resulting in pervasive transcription of chromatin regulatory genes that promote genome-wide histone methylation and acetylation. Efforts to target this tumor-promoting function of mutp53 remain elusive. Here, we therapeutically exploit the GOF mechanisms of p53 codon 158 mutation, a DBD mutant found to be prevalent in lung squamous cell carcinoma. Using high throughput compound screening a...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
Contributors : Li R Kong ; Tuan Z TanSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensMutations in the DNA binding domain (DBD) of TP53 are often associated with the gain-of-function (GOF) of mutp53, resulting in pervasive transcription of chromatin regulatory genes that promote genome-wide histone methylation and acetylation. Efforts to target this tumor-promoting function of mutp53 remain elusive. Here, we therapeutically exploit the GOF mechanisms of p53 codon 158 mutation, a DBD mutant found to be prevalent in lung squamous cell carcinoma. Using high throughput compou...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Homo sapiens Source Type: research
BLM is a multifunctional helicase that plays critical roles in maintaining genome stability. It processes distinct DNA substrates, but not nicked DNA, during many steps in DNA replication and repair. However, how BLM prepares itself for diverse functions remains elusive. Here, using a combined single-molecule approach, we find that a high abundance of BLMs can indeed unidirectionally unwind dsDNA from a nick when an external destabilizing force is applied. Strikingly, human replication protein A (hRPA) not only ensures that limited quantities of BLMs processively unwind nicked dsDNA under a reduced force but also permits t...
Source: eLife - Category: Biomedical Science Tags: Structural Biology and Molecular Biophysics Source Type: research
Wenfa Lu We investigated the potential ability of quercetin to protect against lipopolysaccharide (LPS)-induced intestinal oxidative stress in broiler chickens and the potential role of the Nrf2 (nuclear factor erythroid 2-related factor 2) signaling pathway. One-day-old broiler chickens (n = 240) were randomized into four groups: saline-challenged broiler chickens fed a basal diet (Con), LPS-challenged broiler chickens on a basal diet (LPS), and LPS-treated broiler chickens on a basal diet containing either 200 or 500 mg/kg of quercetin (Que200+LPS or Que500+LPS). Quercetin (200 mg/kg) significantly alleviated LPS-...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
ConclusionsThe data in this paper indicated that WNT7A could be a potential oncogene in OSCC and identified a novel PI3K/AKT/WNT7A/β-catenin/MMP9 signaling for EGF-induced migration of OSCC cells.
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
Journal of Agricultural and Food ChemistryDOI: 10.1021/acs.jafc.9b07046
Source: Journal of Agricultural and Food Chemistry - Category: Food Science Authors: Source Type: research
In this study, the influence of PKM2 on osteogenesis and adipogenesis was assessed in vitro and in vivo. We found that DASA-58 (the activator of PKM2) reduced the enzymatic activity of ALP, and inhibited the levels of osteogenic marker genes, especially RUNX2, which is a crucial transcription factor for osteogenesis. Besides, we provided evidence that C3k, an inhibitor of PKM2, caused increase in mitochondrial membrane potential and maintained low levels of ROS, and promoted mitochondrial fusion. Furthermore, after treatment with DASA-58, the level of active β-catenin gradually decreased, which also inhibited the tran...
Source: Aging - Category: Biomedical Science Authors: Tags: Aging (Albany NY) Source Type: research
CONCLUSIONS: Severe anemia resulted in a disproportionate and persistent increase in intestinal permeability in pre-weaned mice due to the disruption of epithelial adherens junctions. These changes are mediated via microRNA let-7e-mediated depletion of Cdh1 mRNA. PMID: 32090604 [PubMed - as supplied by publisher]
Source: American Journal of Physiology. Gastrointestinal and Liver Physiology - Category: Physiology Authors: Tags: Am J Physiol Gastrointest Liver Physiol Source Type: research
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