Comparison of three multi-cryoprotectant loading protocols for vitrification of porcine articular cartilage.

Comparison of three multi-cryoprotectant loading protocols for vitrification of porcine articular cartilage.
Cryobiology. 2020 Jan 06;:
Authors: Wu K, Shardt N, Laouar L, Chen Z, Prasad V, Elliott JAW, Jomha NM
Abstract
Vitrification is a cryopreservation technique for the long-term storage of viable tissue, but the success of this technique relies on multiple factors. In 2012, our group published a working vitrification protocol for intact human articular cartilage and reported promising chondrocyte recovery after using a four-step multi-cryoprotectant (CPA) loading method that required 570 min. However, this protocol requires further optimization for clinical practice. Herein, we compared three multi-step CPA loading protocols to investigate their impact on chondrocyte recovery after vitrification of porcine articular cartilage on a bone base, including our previous four-step protocol (original: 570 min), and two shorter three-step protocols (optimized: 420 min, and minimally vitrifiable: 310 min). Four different CPAs were used including glycerol, dimethyl sulfoxide, ethylene glycol and propylene glycol. As vitrification containers, two conical tubes (50 ml and 15 ml) were evaluated for their heat transfer impact on chondrocyte recovery after vitrification. Osteochondral dowels were cored into two diameters of 10.0 mm and 6.9 mm with an approximately 10-mm thick bone base, and then allocated into the twelve experime...

Source: Cryobiology - January 5, 2020 Category: Biology Authors: Wu K, Shardt N, Laouar L, Chen Z, Prasad V, Elliott JAW, Jomha NM Tags: Cryobiology Source Type: research