7 ‑MEGA™ 500 regulates the expression of COX‑2, MMP‑3 and type 1 procollagen in UVB‑irradiated human keratinocytes and dermal fibroblasts.

7‑MEGA™ 500 regulates the expression of COX‑2, MMP‑3 and type 1 procollagen in UVB‑irradiated human keratinocytes and dermal fibroblasts. Mol Med Rep. 2019 Dec 20;: Authors: Park YK, Yadav AK, Roshanzadeh A, Ryoo YW, Kim BH, Cha JY, Son YK, Lee NY, Jang BC Abstract AlaskOmega® Omega 7 500, also known as Omega‑7 fatty acid or 7‑MEGA™, is a highly concentrated palmitoleic acid (C16:1). Little is known about how 7‑MEGA regulates skin inflammation and wrinkle formation in cultured skin cells. The present study aimed to investigate the effects of 7‑MEGA on the expression of cyclooxygenase‑2 (COX‑2), matrix metallopeptidase (MMP)‑1/3 and type 1 procollagen, which are markers of skin inflammation and wrinkle formation, in ultraviolet B (UVB)‑irradiated human dermal fibroblasts (HDFs) and keratinocytes (HaCaT). No toxicity was observed upon treatment of HDFs and HaCaT cells with 0.5‑2.5 µl/ml 7‑MEGA. The exposure of HaCaT cells to 10 mJ/cm2 UVB for 6 h resulted in increased protein and/or mRNA expression of COX‑2 and MMP‑3. Treatment of HaCaT cells with 2.5 µl/ml 7‑MEGA suppressed the UVB‑induced expression of COX‑2 and MMP‑3 in these cells. In addition, treatment with 2.5 µl/ml 7‑MEGA attenuated the UVB‑induced expression and phosphorylation levels of c‑Fos and c‑Jun, two components of the activator protein‑1 (AP‑1) transcription factor, in HaCaT cells. Exposure of HDFs to 60 mJ/cm...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research