Serologic detection of antibodies to Brucella spp. using a commercial ELISA in cattle in Grenada, West Indies.
In this study, serum samples from 150 cattle were tested using a commercial competitive enzyme linked immunosorbent assay (SVANOVIR®Brucella-Ab C-ELISA) which detects antibodies to both B. abortus and Brucella melitensis. All cattle tested were greater than 6 months old and were unvaccinated. Sampled cattle were from 35 herds representing animals from all 6 parishes of Grenada. Nine of the 150 animals (6%) were positive for antibodies to B. abortus and/or melitensis by the C-ELISA. Of the 35 herds, 7 (20%) had C-ELISA- positive animals. Three of the 6 parishes contained positive herds. Based on the high sensitivity (98%) and specificity (99.7%) of the C-ELISA, these results strongly indicate the presence of cattle exposed to B. abortus and/or melitensis in Grenada.
PMID: 23959493 [PubMed - in process]
Source: Tropical Biomedicine - Category: Tropical Medicine Authors: Chikweto A, Tiwari K, Kumthekar S, Stone D, Louison B, Thomas D, Sharma R, Hariharan H Tags: Trop Biomed Source Type: research
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