Single cell analysis of β2-Adrenergic receptor dynamics by quantitative fluorescence Microscopy.

CONCLUSION: We describe a highly accurate method of measuring β2-AR surface and total expression based on single cell quantitative immunofluorescence. The method can be used to determine agonist induced desensitization and resensitization process as well receptor kinetics like endocytosis and exocytosis of β2-Adrenergic receptor and can be applied to essentially any other GPCR. PMID: 31840614 [PubMed - as supplied by publisher]
Source: Current Molecular Medicine - Category: Molecular Biology Authors: Tags: Curr Mol Med Source Type: research