Ultrasensitive Biosensing Pathogenic Bacteria by Combining Aptamer-Induced Catalysed Hairpin Assembly Circle Amplification with Microchip Electrophoresis

In this study, a microchip electrophoretic method for biosensing E. coli O157:H7 was developed by using E. coli O157:H7 aptamer (apt-E) for specific bacteria recognition together with aptamer-induced catalysed hairpin assembly (CHA) for significantly improving the sensitivity of bacteria detection. Briefly, three nucleic acid strands (apt-E, hairpin H1, and H2) were used in the CHA amplification. Because different quantities of H1/H2 complexes were formed due to the circle amplification induced with different amounts of apt-E and the correlation between the concentrations of apt-E and E. coli O157:H7, E. coli O157:H7 thus could be quantified by the detection of H1/H2 complexes with microchip electrophoresis (MCE). Under the optimal conditions, the limit of detection was 75 CFU mL-1. This method was also applied to detect E. coli O157:H7 in defatted milk with a satisfying recovery rate. The proposed strategy for E. coli O157:H7 detection is label-free, enzyme-free, ultra-sensitive, and cost-effective. It is also practical and could be applied to detect other bacteria in food samples.
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research

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ConclusionOur findings argue against a co-selection mechanism of antimicrobial drug-resistance and zinc tolerance after dietary zinc supplementation in weaning piglets. An explanation for an increase in multi-drug resistant isolates from piglets with high zinc dietary feeding could be that resistant bacteria to antimicrobial agents are more persistent to stresses such as zinc or copper exposure.
Source: Gut Pathogens - Category: Microbiology Source Type: research
AbstractGiardia muris has been reported from both laboratory and wild rodents in worldwide. During routine care, soft consistency was observed in feces of Swiss albino mice. MotileGiardia spp. trophozoites were observed microscopically on all fecal preparations after stained with Lugol ’s iodine solution. The trophozoites were broadly ovoid in a form with a pointed end. They possess two large nuclei and an adhesive disk whose length overlapped one-half of the trophozoite body length. The caudal flagella are unequal. β giardin (bg) gene region of isolates was successfully amplified and sequenced. According to seq...
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research
Mycobacteria are unique in many aspects of their biology. The development of genetic tools to identify genes critical for their growth by forward genetic analysis holds great promises to advance our understanding of their cellular, physiological and biochemical processes. Here we report the development of a novel transposon, MycoTetOP2, to aid the identification of such genes by direct transposon mutagenesis. This mariner-based transposon contains nested anhydrotetracycline (ATc)-inducible promoters to drive transcription outward from both of its ends. In addition, it includes the Escherichia coli R6Kγ origin to faci...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In conclusion, the detection of two different NDM-5 E. coli strains from a pediatric patient with a history of travel to the Far East countries strongly highlight an increasing trend and risk of importation from such areas.
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
This study sheds light on the virulence and AMR potential of E. coli ST302 strains and informs AMR genomic surveillance.
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In its native environment of rotting vegetation, the soil nematode Caenorhabditis elegans encounters a range of bacteria. This includes species from the ESKAPE group of pathogens that pose a clinical problem in acquired hospital infections. Here, we investigated three Gram-negative members of the ESKAPE group, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Acinetobacter baumannii. Pathogenicity profiles as measured by time to kill adult C. elegans showed that P. aeruginosa was the most pathogenic, followed by K. pneumoniae, while C. elegans cultured on A. baumannii exhibited the same survival as those on the standard l...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Hanks-type kinases encoding genes are present in most cyanobacterial genomes. Despite their widespread pattern of conservation, little is known so far about their role because their substrates and the conditions triggering their activation are poorly known. Here we report that under diazotrophic conditions, normal heterocyst differentiation and growth of the filamentous cyanobacterium Nostoc PCC 7120 require the presence of the Pkn22 kinase, which is induced under combined nitrogen starvation conditions. By analyzing the phenotype of pkn22 mutant overexpressing genes belonging to the regulatory cascade initiating the devel...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Publication date: Available online 20 January 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Adebanjo Najeem Adedoja, Timothy McMahan, John Patrick Neal, Siddhartha Hamal Dhakal, Seetharama Jois, Daniel Romo, Ken Hull, Srinivas GarlapatiAbstractTranslation initiation factor eIF4F is essential for cap-dependent translation initiation in eukaryotes. eIF4F is a trimeric complex consisting of a scaffold protein eIF4G, cap-binding protein eIF4E and DEAD-box RNA helicase eIF4A. eIF4F binds to the 5’ cap structure of the mRNA through eIF4E and facilitates the binding of the preinitiation complex (PIC) via prot...
Source: Molecular and Biochemical Parasitology - Category: Parasitology Source Type: research
Bacteremia is an uncommon complication associated with Shigella infections. What are the risk factors for this rare infection?Emerging Infectious Diseases
Source: Medscape Hiv-Aids Headlines - Category: Infectious Diseases Tags: Infectious Diseases Journal Article Source Type: news
Contributors : Martin A White ; Elise Darmon ; Manuel A Lopez-Vernaza ; David LeachSeries Type : OtherOrganism : Escherichia coli K-12The effect of a site-specific DNA double-strand break on the abundance of E. coli chromosomal DNA during exponential growth was investigated by marker frequency analysis (MFA).
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Other Escherichia coli K-12 Source Type: research
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