Plasma metabolomics analysis of endogenous and exogenous metabolites in the rat after administration of Lonicerae Japonicae Flos.

Plasma metabolomics analysis of endogenous and exogenous metabolites in the rat after administration of Lonicerae Japonicae Flos. Biomed Chromatogr. 2019 Dec 08;:e4773 Authors: Li L, Ma S, Wang D, Chen L, Wang X Abstract Lonicerae Japonicae Flos (LJF) is a typical herbal medicine, and further developed into functional food. LJF has various biological activities with complicated chemical components. The global metabolomics, focusing on both the endogenous and exogenous metabolites, have not yet been investigated for LJF on normal healthy rats with liquid chromatography-mass spectrometry. In the study, plasma metabolomics analysis was performed out with different administration time, and the exogenous metabolites were identified. Partial least squares discriminant analysis showed specific difference in the dosed rats. Cholic acid, indoleacrylic acid, indolelactic acid and hippuric acid, N-acetyl-phenylalanine and N-acetyl-serotonin were significantly accumulated in the dosed rats. Lyso phosphatidylethanolamine and lyso phosphatidylcholine including plasmalogen were increased. There were 25 components of LJF annotated, including 15 prototypes and 10 metabolites. The 15 prototypes included phenolic acids, flavonoids and iridoids, and contents of them were decreased with the administration time. Glucuronidation and sulfation of polyphenols were found for LJF. The exogenous glucuronide and sulfate metabolites were identified with neutral l...
Source: Biomedical Chromatography : BMC - Category: Biomedical Science Authors: Tags: Biomed Chromatogr Source Type: research