Synergistic Effect of Reductase and Keratinase for Facile Synthesis of Protein Coated Gold Nanoparticles.

Synergistic Effect of Reductase and Keratinase for Facile Synthesis of Protein Coated Gold Nanoparticles. J Microbiol Biotechnol. 2014 Dec 12; Authors: Gupta S, Singh SP, Singh R Abstract We have synthesized gold nanoparticles (GNPs) using chicken feather (poultry waste) and Bacillus subtilis RSE163. Disulphide reductase and keratinase produced by Bacillus subtilis during the degradation of chicken feather has been used to reduce Au(3+) form HAuCl4 precursor to produce gold nanoparticles. The synthesized biogenic GNPs were characterized by UV-Visible Spectroscopy, Transmission Electron Microscopy (TEM) and Zeta potential measurements. Fourier transform infrared (FTIR) spectroscopy indicates the presence of protein capping on as synthesized GNPs imparting multi-functionality to GNPs surface. Further, the nontoxic nature of biogenic GNPs was insured by interaction with Escherichia coli (ATCC11103), where TEM images and enhancement of growth rate of E.coli in log phase signifies their nontoxic nature. Results indicate the synthesis of biocompatible GNPs using poultry waste may find their potential applications in drug delivery and sensing. PMID: 25502826 [PubMed - as supplied by publisher]
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Tags: J Microbiol Biotechnol Source Type: research