Efficient inhibition of foot-and-mouth disease virus replication in vitro by artificial microRNA targeting 3D polymerase.

In this study, the efficacy of amiRNAs in preventing FMD virus replication in a permissive cell culture system was investigated, by generating stable cell lines expressing amiRNAs targeting three functional regions of the FMD virus (FMDV) genome (IRES, 3B3 and 3D). The results showed that amiRNA targeting 3D polymerase is relatively more efficient. However, expression of multiple microRNAs targeting the three regions did not exhibit additive effect. The data suggest that 3D specific miRNA is a potential valid strategy in developing novel antiviral measures against FMDV infection. Keywords: artificial microRNA; foot-and-mouth disease virus; virus inhibition.
PMID: 31802691 [PubMed - in process]

Source: Acta Virologica - December 6, 2019 Category: Infectious Diseases Authors: Basagoudanavar SH, Ranjitha HB, Hosamani M, Kolangath SM, Selvan RPT, Sreenivasa BP, Saravanan P, Sanyal A, Venkataramanan R Tags: Acta Virol Source Type: research