Investigating the InsP3 Receptor in Living Cells by Caged InsP3.

Investigating the InsP3 Receptor in Living Cells by Caged InsP3. Methods Mol Biol. 2020;2091:121-129 Authors: Hui X, Lipp P Abstract The inositol 1,4,5-trisphosphate receptor (InsP3R) operates as an intracellular ligand-gated Ca2+ channel and plays a pivotal role in cellular Ca2+ homeostasis across all living cells. It is activated following membrane receptor-ligand interactions and stimulation of subsequent signaling cascades involving the enzymatic breakdown of the membrane lipid phosphatidyl-4,5-bisphosphate (PIP2) into the membrane-delimited second messenger diacylglycerol (DAG) and the diffusible second messenger inositol-1,4,5-trisphophate (InsP3). Modulation of InsP3R's activity is thus involved in a plethora of physiological and pathological processes. Here we combine membrane permeable photoactive caged-InsP3 with Ca2+ imaging techniques in living cells to study the channel's in vivo properties. Using UV-flashes of variable energy, the activity properties of InsP3R can be investigated in great detail in its native environment. PMID: 31773575 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research