Metabonomic analysis of potential plasma biomarkers in pyrexia rats after treatment with Gegenqinlian decoction, aspirin and itraconazole by UHPLC-FT-ICR-MS

Publication date: Available online 27 November 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Ting Liu, Ruiyun Li, Yue Cui, Zhiguo Yu, Yunli ZhaoAbstractA metabonomic approach involving an ultrahigh-performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS) was used to investigate the changes in the endogenous metabolites in the plasma of rats with yeast-induced pyrexia treated with Gegenqinlian decoction (GQLD), aspirin and itraconazole. The differences in the small molecule profiles of treatment using Traditional Chinese medicine, etiological treatment and symptomatic treatment were elucidated. Thirty-six plasma metabolites of the anti-pyrexia effect of GQLD, aspirin and itraconazole were identified or putatively identified, and 20 metabolites were restored to their normal levels in the three treated groups. Their metabolic pathways, indicated that GQLD, aspirin and itraconazole ameliorated the rats with yeast-induced pyrexia predominantly by regulating the metabolisms of phospholipid, sphingolipid, fatty acid oxidation, fatty acid amides, amino acid and glycerolipid in vivo. The pharmacodynamics and metabolomic results showed that the three medicines exhibited the therapeutic effect on pyrexia by regulating the perturbations of multiple metabolisms. The study provided a scientific basis for the in-depth understanding of the therapeutic effects of GQLD, aspirin and itraconazole on rats with yeast-i...
Source: Journal of Pharmaceutical Analysis - Category: Drugs & Pharmacology Source Type: research