Thermodynamics of clay – Drug complex dispersions: Isothermal titration calorimetry and high-performance liquid chromatography
Publication date: Available online 5 December 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Ana-Maria Totea, Juan Sabin, Irina Dorin, Karl Hemming, Peter R. Laity, Barbara R. Conway, Laura Waters, Kofi Asare-AddoAbstractAn understanding of the thermodynamics of the complexation process utilized in sustaining drug release in clay matrices is of great importance. Several characterisation techniques as well as isothermal calorimetry were utilized in investigating the adsorption process of a model cationic drug (diltiazem hydrochloride, DIL) onto a pharmaceutical clay system (magnesium aluminium silicate, MAS). X-ra...
Source: Journal of Pharmaceutical Analysis - December 6, 2019 Category: Drugs & Pharmacology Source Type: research

Comparing different domains of analysis for the characterisation of N-Glycans on monoclonal antibodies
In this study, ten different analytical workflows for N-glycan analysis were compared on four therapeutic monoclonal antibodies. While observing good comparability between the quantitative results generated, it was possible to appreciate the advantages and disadvantages of each technique and to summarise all the observations to guide the choice of the most appropriate analytical workflow according to application and the desired depth of data generated.Graphical abstract (Source: Journal of Pharmaceutical Analysis)
Source: Journal of Pharmaceutical Analysis - November 30, 2019 Category: Drugs & Pharmacology Source Type: research

GC-NICI-MS analysis of acetazolamide and other sulfonamide (R-SO2-NH2) drugs as pentafluorobenzyl derivatives [R-SO2-N(PFB)2] and quantification of pharmacological acetazolamide in human urine
Publication date: Available online 28 November 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Olga Begou, Kathrin Drabert, Georgios Theodoridis, Dimitrios TsikasAbstractAcetazolamide (molecular mass (MM), 222) belongs to the class of sulfonamides (R-SO2-NH2) and is one of the strongest pharmacological inhibitors of carbonic anhydrase activity. Acetazolamide is excreted unchanged in the urine. Here, we report on the development, validation and biomedical application of a stable-isotope dilution GC-MS method for the reliable quantitative determination of acetazolamide in human urine. The method is based on evaporat...
Source: Journal of Pharmaceutical Analysis - November 29, 2019 Category: Drugs & Pharmacology Source Type: research

Metabonomic analysis of potential plasma biomarkers in pyrexia rats after treatment with Gegenqinlian decoction, aspirin and itraconazole by UHPLC-FT-ICR-MS
Publication date: Available online 27 November 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Ting Liu, Ruiyun Li, Yue Cui, Zhiguo Yu, Yunli ZhaoAbstractA metabonomic approach involving an ultrahigh-performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS) was used to investigate the changes in the endogenous metabolites in the plasma of rats with yeast-induced pyrexia treated with Gegenqinlian decoction (GQLD), aspirin and itraconazole. The differences in the small molecule profiles of treatment using Traditional Chinese medicine, etiological tr...
Source: Journal of Pharmaceutical Analysis - November 27, 2019 Category: Drugs & Pharmacology Source Type: research

Comparative analysis of constitutes and metabolites for traditional Chinese medicine using IDA and SWATH data acquisition modes on LC-Q-TOF MS
Publication date: Available online 19 November 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Dian Kang, Qingqing Ding, Yangfan Xu, Xiaoxi Yin, Huimin Guo, Tengjie Yu, He Wang, Wenshuo Xu, Guangji Wang, Yan LiangAbstractIdentification of components and metabolites of traditional Chinese medicines (TCMs) employing liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-QTOF MS) techniques with information-dependent acquisition (IDA) approaches is more and more frequent. A current drawback of IDA-MS is that the complexity of a sample might prevent important compounds from being triggered in IDA settin...
Source: Journal of Pharmaceutical Analysis - November 20, 2019 Category: Drugs & Pharmacology Source Type: research

Nuclear magnetic resonance based structure of the protoberberine alkaloid coralyne and its self-association by spectroscopy techniques
Publication date: Available online 26 September 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Kumar Padmapriya, Ritu BarthwalAbstractCoralyne is an important alkaloid due to its anti-cancer and other medicinal properties. It targets DNA in cells and acts as human topoisomerase-I poison, telomerase inhibitor and nucleic acid intercalator. It has high tendency to undergo self-association, which is a matter of concern for therapeutic applications. The understanding of its interaction with DNA requires precise knowledge of chemical shifts in Nuclear Magnetic Resonance (NMR) spectra besides self-association. The pres...
Source: Journal of Pharmaceutical Analysis - November 17, 2019 Category: Drugs & Pharmacology Source Type: research

Isoflavones influencing on pharmacokinetic profiles of main iridoids from Gardeniae Fructus in rats
Publication date: Available online 14 November 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Ruirui Chang, Jialin Liu, Taohong Huang, Qiang Li, Jun Wen, Weidong Chen, Tingting ZhouAbstractGardeniae Fructus (GF) and Semen Sojae Praeparatum (SSP) are both medicine food homologies, and widely used in Chinese clinical prescriptions together. The research investigated the pharmacokinetics of four iridoids in normal rats and isolfavones-feeding rats, which were administrated with isolfavones from SSP for 7, 14, 21 and 28 consecutive days. A validated LC-MS/MS method was developed for determining shanzhiside, genipin-1...
Source: Journal of Pharmaceutical Analysis - November 14, 2019 Category: Drugs & Pharmacology Source Type: research

Vancomycin pretreatment attenuates acetaminophen-induced liver injury through 2-hydroxybutyric acid
Publication date: Available online 6 November 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Ningning Zheng, Yu Gu, Ying Hong, Lili Sheng, Linlin Chen, Feng Zhang, Zimiao Weng, Weidong Zhang, Zean Zhang, Wei Jia, Houkai LiAbstractLiver injury caused by acetaminophen (AP) overdose is a leading public health problem. Although AP-induced liver injury is well recognized as the formation of N-acetyl-p-benzoquinone (NAPQI), a toxic metabolite of AP, resulting in cell damage, emerging evidence indicates that AP-induced liver injury is also associated with gut microbiota. However, the gut microbiota-involved mechanism re...
Source: Journal of Pharmaceutical Analysis - November 8, 2019 Category: Drugs & Pharmacology Source Type: research

Plant-derived secondary metabolites as the main source of efflux pump inhibitors and methods for identification
Publication date: Available online 5 November 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Armel Jackson Seukep, Victor Kuete, Lutfun Nahar, Satyajit D. Sarker, Mingquan GuoAbstractThe upsurge of multiple drug resistance (MDR) bacteria substantially diminishes the effectiveness of antibiotic arsenal and therefore intensifies the rate of therapeutic failure. The major factor in MDR is efflux pump-mediated resistance. A unique pump can make bacteria withstanding to a wide range of structurally diverse compounds. Therefore, their inhibition is a promising route to eliminate resistance phenomenon in bacteria. Phyto...
Source: Journal of Pharmaceutical Analysis - November 7, 2019 Category: Drugs & Pharmacology Source Type: research

Offline two-dimensional liquid chromatography coupled with ion mobility-quadrupole time-of-flight mass spectrometry enabling four-dimensional separation and characterization of the multicomponents from white ginseng and red ginseng
Publication date: Available online 3 November 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Tiantian Zuo, Chunxia Zhang, Weiwei Li, Hongda Wang, Ying Hu, Wenzhi Yang, Li Jia, Xiaoyan Wang, Xiumei Gao, Dean GuoAbstractInherent complexity of plant metabolites necessitates the use of multi-dimensional information to accomplish comprehensive profiling and confirmative identification. A dimension-enhanced strategy, by offline two-dimensional liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry (2D-LC/IM-QTOF-MS) enabling four-dimensional separations (2D-LC, IM, and MS), is proposed. In combi...
Source: Journal of Pharmaceutical Analysis - November 4, 2019 Category: Drugs & Pharmacology Source Type: research

Enantioseparation of FMOC-amino acids using an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine-silica hybrid monolithic column by nano-LC. Quantitation of lL-norvaline and lL-tryptophan in dietary supplements
Publication date: Available online 23 October 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Dongsheng Xu, Elena Sánchez-López, Qiqin Wang, Zhengjin Jiang, María Luisa MarinaAbstractAn analytical methodology based on an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine (MQD)-silica hybrid monolithic column was developed for the enantioseparation of 9-fluorenylmethoxycarbonyl (FMOC) derivatized amino acids by nano-liquid chromatography. The mobile phase was optimized including the apparent pH, content of ACN, and concentration of the buffer to obtain a satisfactory enantioresolutio...
Source: Journal of Pharmaceutical Analysis - October 24, 2019 Category: Drugs & Pharmacology Source Type: research

Editorial Board
Publication date: October 2019Source: Journal of Pharmaceutical Analysis, Volume 9, Issue 5Author(s): (Source: Journal of Pharmaceutical Analysis)
Source: Journal of Pharmaceutical Analysis - October 12, 2019 Category: Drugs & Pharmacology Source Type: research

Erucic acid from Isatis indigotica Fort. Suppresses influenza A virus replication and inflammation in vitro and in vivo through modulation of NF-κB and p38 MAPK pathway
Publication date: Available online 4 October 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Xiaoli Liang, Yuan Huang, Xiping Pan, Yanbing Hao, Xiaowei Chen, Haiming Jiang, Jing Li, Beixian Zhou, Zifeng YangAbstractIsatis indigotica Fort. (Ban-Lan-Gen) is an herbal medicine prescribed for influenza treatment. However, its active components and mode of action remain mostly unknown. In the present study, erucic acid was isolated from Isatis indigotica Fort., and subsequently its underlying mechanism against influenza A virus (IAV) infection was investigated in vitro and in vivo. Our results demonstrated that erucic ...
Source: Journal of Pharmaceutical Analysis - October 5, 2019 Category: Drugs & Pharmacology Source Type: research

Estimation of boswellic acids in herbal formulations containing Boswellia serrata extract and comprehensive characterization of secondary metabolites using UPLC-Q-Tof-MSe
In this study quantification of boswellic acids with UPLC-PDA method was performed as per the pharmacopeia guide lines. Furthermore, minor phytochemical constituents were identified and characterized with the help of LC-Q-Tof-MS/MS fragmentation data and various isoforms of boswellic acids and tirucallic acids in B. serrata oleo-gum-resin extract were identified.Graphical abstract (Source: Journal of Pharmaceutical Analysis)
Source: Journal of Pharmaceutical Analysis - October 2, 2019 Category: Drugs & Pharmacology Source Type: research

Nanodiamonds with powerful ability for drug delivery and biomedical applications: Recent updates on in vivo study and patents
Publication date: Available online 1 October 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Swati Chauhan, Neha Gulati Jain, Upendra NagaichAbstractNanodiamonds are novel nanosized carbon building blocks possessing varied fascinating mechanical, chemical, optical and biological properties making them significant active moiety carriers for biomedical application. These are known as the most ‘captivating’ crystals attributed to their chemical inertness and unique properties posing them useful for variety of applications in biomedical era. Alongside, it becomes increasingly important to find, ascertain a...
Source: Journal of Pharmaceutical Analysis - October 2, 2019 Category: Drugs & Pharmacology Source Type: research

Nuclear magnetic resonance based structure of the protoberberine alkaloid coralyne and its self association by spectroscopy techniques
Publication date: Available online 26 September 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Kumar Padmapriya, Ritu BarthwalAbstractCoralyne is an important alkaloid due to its anti cancer and other medicinal properties. It targets DNA in cells and acts as human topoisomerase-I poison, telomerase inhibitor and nucleic acid intercalator. It has high tendency to undergo self association, which is a matter of concern for therapeutic applications. The understanding of its interaction with DNA requires precise knowledge of chemical shifts in Nuclear Magnetic Resonance (NMR) spectra besides self association. The pres...
Source: Journal of Pharmaceutical Analysis - September 27, 2019 Category: Drugs & Pharmacology Source Type: research

Potential synergic mechanism of Wutou-Gancao herb-pair by inhibiting efflux transporter P-gp
In this study, potential synergic mechanism of the herb pair was investigated by utilizing multiple approaches. In silico and in vitro Caco-2 cell model were applied to study the potential binding mode of bioactive ingredients existing in liquorice with P-gp, as well as the inhibition effects on P-gp. Additionally, anti-inflammatory activity of aconitine (AC) combined with active ingredients of liquorice, as well as pharmacokinetic patterns of AC after co-administration were investigated. Anti-inflammatory effect of AC (1 mg/kg) in rats was enhanced in combination with bioactive ingredients of liquorice (10 mg/kg). I...
Source: Journal of Pharmaceutical Analysis - September 27, 2019 Category: Drugs & Pharmacology Source Type: research

Development of a UHPLC-MS/MS method for the quantification of ilaprazole enantiomers in rat plasma and its pharmacokinetic application
In this study, a specific and sensitive LC-MS/MS method has been developed and validated, for the quantification of ilaprazole enantiomers in the rat plasma, using R-lansoprazole as the internal standard. The enantioseparation was achieved on a CHIRALPAK AS-RH column (4.6 mm × 150 mm, i.d. 5 μm), with a mobile phase composed of 10 mM ammonium acetate aqueous solution and acetonitrile (60:40, v/v), at a flow-rate of 0.5 mL/min. The method was validated over the concentration range of 0.5–300 ng/mL for both, R- and S -ilaprazole. The lower limit of quantification was 0.5 ng/mL for both enanti...
Source: Journal of Pharmaceutical Analysis - September 19, 2019 Category: Drugs & Pharmacology Source Type: research

Trace determination and characterization of ginsenosides in rat plasma through magnetic dispersive solid-phase extraction based on core-shell polydopamine-coated magnetic nanoparticles
This study presented a one-pot synthesis of magnetic polydopamine nanoparticles (Fe3O4@SiO2@PDA NPs) with multiple recognition sites for the magnetic dispersive solid-phase extraction (MDSPE) of ginsenosides from rat plasma treated with white ginseng. The extracted ginsenosides were characterized by combining an ultra-high-performance liquid chromatography coupled to a high-resolution mass spectrometry with supplemental UNIFI libraries. Response surface methodology was statistically used to optimize the extraction procedure of the ginsenosides. The reusability of Fe3O4@SiO2@PDA NPs was also examined and the results showed ...
Source: Journal of Pharmaceutical Analysis - September 6, 2019 Category: Drugs & Pharmacology Source Type: research

FITC labeling of human insulin and transport of FITC-Insulin conjugates through MDCK cell monolayer
Publication date: Available online 29 August 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Darshana Shah, Yuxing Guo, Joseph Ocando, Jun ShaoAbstractFluorescein isothiocyanate labeled insulin (FITC-Insulin) has been widely used for bioanalytical applications. Due to the high cost of commercial FITC-Insulin and tedious labeling procedures described in the literature, there is still a need to develop a cost effective, reliable and quick labeling method for insulin. The purpose of the present work was to develop a quick and affordable method for FITC labeling of human insulin and to determine the effect of differen...
Source: Journal of Pharmaceutical Analysis - August 30, 2019 Category: Drugs & Pharmacology Source Type: research

Quantitative analysis of 3-isopropylamino-1,2-propanediol as a degradation product of metoprolol in pharmaceutical dosage forms by HILIC-CAD
Publication date: Available online 28 August 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Qun Xu, Shane TanAbstractAryloxypropanolamine is an essential structural scaffold for a variety of β-adrenergic receptor antagonists such as metoprolol. Molecules with such a structural motif tend to degrade into α, β–hydroxypropanolamine impurities via a radical–initiated oxidation pathway. These impurities are typically polar and nonchromophoric, and are thus often overlooked using traditional reversed phase chromatography and UV detection. In this work, stress testing of metoprolol confirmed ...
Source: Journal of Pharmaceutical Analysis - August 29, 2019 Category: Drugs & Pharmacology Source Type: research

Editorial Board
Publication date: August 2019Source: Journal of Pharmaceutical Analysis, Volume 9, Issue 4Author(s): (Source: Journal of Pharmaceutical Analysis)
Source: Journal of Pharmaceutical Analysis - August 14, 2019 Category: Drugs & Pharmacology Source Type: research

Preface for advances in pharmaceutical analysis 2018
Publication date: August 2019Source: Journal of Pharmaceutical Analysis, Volume 9, Issue 4Author(s): (Source: Journal of Pharmaceutical Analysis)
Source: Journal of Pharmaceutical Analysis - August 14, 2019 Category: Drugs & Pharmacology Source Type: research

Karacoline, identified by network pharmacology, reduces degradation of the extracellular matrix in intervertebral disc degeneration via the NF-κB signaling pathway
In this study, we selected key matrix metalloproteinases (MMPs), collagen II, and aggrecan as targets due to their association with intervertebral disc degeneration (IDD). Using these targets, we then used network pharmacology to predict a series of molecules that might exert therapeutic effects on IDD. Of these molecules, karacoline was predicted to have the best effect. Tumor necrosis factor (TNF)-α is known to promote the degeneration of the extracellular matrix in IDD. We therefore applied different concentrations of karacoline (0, 1.25, or 12.88 μM) along with 100 ng/mL TNF-α to rat nucleus pulposus...
Source: Journal of Pharmaceutical Analysis - July 26, 2019 Category: Drugs & Pharmacology Source Type: research

Irbesartan desmotropes: Solid-state characterization, thermodynamic study and dissolution properties
Publication date: Available online 10 July 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Andrea Mariela Araya-Sibaja, Carlos Eduardo Maduro Campos, Cinira Fandaruff, José Roberto Vega-Baudrit, Teodolito Guillén-Girón, Mirtha Navarro Hoyos, Silvia Lucía CuffiniAbstractIrbesartan (IBS) is a tetrazole derivative and antihypertensive drug that has two interconvertible structures, 1H- and 2H-tautomers. The difference between them lies in the protonation of the tetrazole ring. In the solid-state, both tautomers can be isolated as crystal forms A (1H-tautomer) and B (2H-tautomer). Studies ha...
Source: Journal of Pharmaceutical Analysis - July 10, 2019 Category: Drugs & Pharmacology Source Type: research

Temperature and eluent composition effects on enantiomer separation of carvedilol by high-performance liquid chromatography on immobilized amylose-based chiral stationary phases
Publication date: Available online 4 April 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Cristina Panella, Rosella Ferretti, Adriano Casulli, Roberto CirilliAbstractCarvedilol is a chiral drug with potent antihypertensive and antianginal activities. Although it is clinically used as a racemic mixture, its enantiomers show different pharmacokinetic and pharmacodynamic profiles. Here, the direct chiral separation of racemic drug by high performance liquid chromatography using two immobilized-type amylose-based chiral stationary phases is presented. Some chromatographic parameters, such as retention and selectivity...
Source: Journal of Pharmaceutical Analysis - June 28, 2019 Category: Drugs & Pharmacology Source Type: research

Determination of iohexol by capillary blood microsampling and UHPLC-MS/MS
This study proposes and evaluates a new LC-MS/MS method for the iohexol determination from capillary blood, prelevated using volumetric absorbative microsampling systems (VAMS). As an alternative to VAMS a brand new HemaPEN® device for micro-prelevation was also tested.A new high throughput sample preparation protocol adapted for Iohexol quantification from whole blood VAMS samples was developed. The medium term stability study of iohexol in dried whole blood VAMS samples that was conducted showed a good stability of this molecule for up to 12 days.By collecting only 10 μL of blood, iohexol can be analyzed from dr...
Source: Journal of Pharmaceutical Analysis - June 28, 2019 Category: Drugs & Pharmacology Source Type: research

Comparative analysis of twenty-five compounds in different parts of Astragalus membranaceus var. mongholicus and Astragalus membranaceus by UPLC-MS/MS
Publication date: Available online 25 June 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Yuan Li, Sheng Guo, Yue Zhu, Hui Yan, Da-wei Qian, Han-qing Wang, Jian-qiang Yu, Jin-ao DuanAbstractAs a traditional Chinese medicine, the root of Astragalus membranaceus var. mongholicus (AMM) or A. membranaceus (AM) has been widely used in China and other Asian countries for thousands of years. Till now, the flavonoids, phenolic acids and saponins are considered as the main active components contributed to their therapeutic effect in these plants. In order to clarify the distribution and contents of these compounds in diff...
Source: Journal of Pharmaceutical Analysis - June 26, 2019 Category: Drugs & Pharmacology Source Type: research

Analytical methodologies for determination of methotrexate and its metabolites in pharmaceutical, biological and environmental samples
Publication date: Available online 20 June 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Forough Karami, Sara Ranjbar, Younes Ghasemi, Monica NegahdaripourAbstractMethotrexate (MTX) is a folate antagonist drug used for several diseases, such as cancers, various malignancies, rheumatoid arthritis (RA) and inflammatory bowel disease. Due to its structural features, including the presence of two carboxylic acid groups and its low native fluorescence, there are some challenges to develop analytical methods for its determination. MTX is metabolized to 7-hydroxymethotrexate (7-OH-MTX), 2,4-diamino-N10-methylpteroic ac...
Source: Journal of Pharmaceutical Analysis - June 22, 2019 Category: Drugs & Pharmacology Source Type: research

Editorial Board
Publication date: June 2019Source: Journal of Pharmaceutical Analysis, Volume 9, Issue 3Author(s): (Source: Journal of Pharmaceutical Analysis)
Source: Journal of Pharmaceutical Analysis - June 20, 2019 Category: Drugs & Pharmacology Source Type: research

Elucidating the interaction of kansui and licorice by comparative plasma/tissue metabolomics and a heatmap with relative fold change
Publication date: Available online 1 June 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Yan-Yan Chen, Juan Shen, Yu-Ping Tang, Jin-Gao Yu, Jing Wang, Shi-Jun Yue, Jie Yang, Jia-Qian Chen, Li-Mei Feng, Zhen-Hua Zhu, Wei-Wei Tao, Li Zhang, Jin-Ao DuanAbstractAlthough compatibility is highly advocated in traditional Chinese medicine (TCM), inappropriate combination of some herbs may reduce the therapeutic action and even produce toxic effects. Kansui and licorice, one of TCM “Eighteen Incompatible Medicaments”, are the most representative cases of improper herbal combination, which may still be applied ...
Source: Journal of Pharmaceutical Analysis - June 1, 2019 Category: Drugs & Pharmacology Source Type: research

Research advances in the detection of miRNA
Publication date: Available online 25 May 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Jiawei Ye, Mingcheng Xu, Xueke Tian, Sheng Cai, Su ZengAbstractMicroRNAs (miRNAs) are a family of endogenous, small (approximately 22 nucleotides in length), noncoding, functional RNAs. With the development of molecular biology, the research of miRNA biological function has attracted significant interest, as abnormal miRNA expression is identified to contribute to serious human diseases such as cancers. Traditional methods for miRNA detection do not meet current demands. In particular, nanomaterial-based methods, nucleic acid...
Source: Journal of Pharmaceutical Analysis - May 26, 2019 Category: Drugs & Pharmacology Source Type: research

Essential oils of Origanum compactum increase membrane permeability, disturb cell membrane integrity, and suppress quorum-sensing phenotype in bacteria
Publication date: Available online 7 March 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Abdelhakim Bouyahya, Jamal Abrini, Nadia Dakka, Youssef BakriAbstractThe aim of this study was to investige antibacterial activity of Origanum compactum essential oils collected at three phenological stages on Escherichia coli and Bacillus subtilis. The antibacterial activity was evaluated using the agar-well diffusion assay. The MIC and MBC values were determined using the micro-dilution assay. The investigation of the antibacterial action was carried out by the evaluation of the effect of O. compactum essential oils o...
Source: Journal of Pharmaceutical Analysis - May 25, 2019 Category: Drugs & Pharmacology Source Type: research

Rapid identification of chemical profile in Gandou decoction by UPLC-Q-TOF-MSE coupled with novel informatics UNIFI platform
This study developed a rapid method for characterizing the chemical constituents in GDD, which could not only be used for chemical standardization and quality control, but also be helpful for further research of GDD in vivo. (Source: Journal of Pharmaceutical Analysis)
Source: Journal of Pharmaceutical Analysis - May 16, 2019 Category: Drugs & Pharmacology Source Type: research

Development and validation of an LC-MS/MS method for tyrphostin A9
Publication date: Available online 8 March 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Lyndsey F. Meyer, Dhaval K. ShahAbstractHere we have presented a sensitive and selective LC-MS/MS method for the quantification of tyrphostin A9, which is a selective inhibitor for platelet derived growth factor receptor tyrosine kinase and has been investigated in vitro as a potent oxidative phosphorylation uncoupler. The murine analytical method was developed for three biological matrices: cell culture media, adipose 3T3-L1 cell lysate, and mouse plasma. For each matrix the limit of detection and the limit of quantificatio...
Source: Journal of Pharmaceutical Analysis - May 15, 2019 Category: Drugs & Pharmacology Source Type: research

Prospects to the formation and control of potential dimer impurity E of pantoprazole sodium sesquihydrate
Publication date: Available online 1 March 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Arun Kumar Awasthi, Lalit Kumar, Punit Tripathi, Madhava Golla, Cirandur Suresh Reddy, Pramod KumarAbstractPantoprazole sodium, a substituted benzimidazole derivative, is an irreversible proton pump inhibitor which is primarily used for the treatment of duodenal ulcers, gastric ulcers, and gastroesophageal reflux disease (GERD). The monographs of European Pharmacopoeia (Ph. Eur.) and United States Pharmacopoeia (USP) specify six impurities, viz.; impurities A, B, C, D, E and F, respectively for its active pharmaceutical ingr...
Source: Journal of Pharmaceutical Analysis - May 14, 2019 Category: Drugs & Pharmacology Source Type: research

Effect of an extraction solvent on the antioxidant quality of Pinus densiflora needle extract
Publication date: Available online 15 March 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Thamizhiniyan Venkatesan, Young-Woong Choi, Young-Kyoon KimAbstractPinus densiflora needle extract (PDNE) is widely reported to have many pharmacological activities including antioxidant potential. However, the solvent system used for extraction greatly affects its antioxidant quality. Hence, in the present study, we investigated the effect of a different ratio (vol/vol) of ethanol to water (0–100%) in the extraction of PDNE with potent antioxidant capacity. The chemical assays, 2,2-diphenyl-1 picrylhydrazyl (DPPH) an...
Source: Journal of Pharmaceutical Analysis - May 14, 2019 Category: Drugs & Pharmacology Source Type: research

Advances in capillary electro-chromatography
Publication date: Available online 11 May 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Zhenkun Mao, Zilin ChenAbstractCapillary electrochromatography (CEC) is a micro-scale separation technique which is a hybrid between capillary electrophoresis (CE) and liquid chromatography (LC). CEC can be performed in packed, monolithic and open-tubular columns. In recent three years (from 2016 to 2018), enormous attention for CEC is the development of novel stationary phases. This review mainly covers the development of novel stationary phases for open-tubular and monolithic columns. In particular, some biomaterials attrac...
Source: Journal of Pharmaceutical Analysis - May 12, 2019 Category: Drugs & Pharmacology Source Type: research

Simultaneous determination of amino acids in different teas using supercritical fluid chromatography coupled with single quadrupole mass spectrometry
In this study, a novel method based on supercritical fluid chromatography coupled with mass spectrometry (SFC-MS) was developed to simultaneously determine 11 amino acids in different types of tea (green teas, Oolong tea, black tea and Pu-erh tea). The separation Conditions for the analysis of the selected amino acids including the column type, temperature and backpressure as well as the type of additive, were carefully optimized. The best separation of the 11 amino acids was obtained by adding water (5%, v/v) and trifluoroacetic acid (0.4%, v/v) to the organic modifier (methanol). Finally, the developed SFC-MS method was ...
Source: Journal of Pharmaceutical Analysis - May 11, 2019 Category: Drugs & Pharmacology Source Type: research

Carbon nanotubes: Evaluation of toxicity at biointerfaces
Publication date: Available online 25 April 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Debashish Mohanta, Soma Patnaik, Sanchit Sood, Nilanjan DasAbstractCarbon nanotubes (CNTs) are a class of carbon allotropes with interesting properties that make them productive materials for usage in various disciplines of nanotechnology such as in electronics equipments, optics and therapeutics. They exhibit distinguished properties viz., strength, and high electrical and heat conductivity. Their uniqueness can be attributed due to the bonding pattern present between the atoms which are very strong and also exhibit high e...
Source: Journal of Pharmaceutical Analysis - April 26, 2019 Category: Drugs & Pharmacology Source Type: research

Pharmacokinetics and enterohepatic circulation of jervine, an antitumor steroidal alkaloid from Veratrum nigrum in rats
Publication date: Available online 25 April 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Bingjing Zheng, Caihong Wang, Wenwen Song, Xiaoxia Ye, Zheng XiangAbstractJervine is a novel steroidal alkaloid from Veratrum nigrum L. exhibiting both antitumor effect and potential toxicity. The aim of study was to characterize the pharmacokinetic behaviors and enterohepatic circulation of jervine in rats. A rapid and simple ultra-high performance liquid chromatography-tandem mass spectrometric method was developed and validated for quantification of jervine and alpinetin (internal standard) in rat plasma. After extractio...
Source: Journal of Pharmaceutical Analysis - April 26, 2019 Category: Drugs & Pharmacology Source Type: research

Editorial Board
Publication date: April 2019Source: Journal of Pharmaceutical Analysis, Volume 9, Issue 2Author(s): (Source: Journal of Pharmaceutical Analysis)
Source: Journal of Pharmaceutical Analysis - April 12, 2019 Category: Drugs & Pharmacology Source Type: research

Temperature and eluent composition effects on enantiomer separation of carvedilol by high‐performance liquid chromatography on immobilized amylose-based chiral stationary phases
Publication date: Available online 4 April 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Cristina Panella, Rosella Ferretti, Adriano Casulli, Roberto CirilliAbstractCarvedilol is a chiral drug with potent antihypertensive and antianginal activity. Although it is clinically used as a racemic mixture, its enantiomers show different pharmacokinetic and pharmacodynamic profiles. Here, the direct chiral separation of racemic drug by high performance liquid chromatography using two immobilized-type amylose-based chiral stationary phases is presented. Some chromatographic parameters, such as retention and selectivity, ...
Source: Journal of Pharmaceutical Analysis - April 5, 2019 Category: Drugs & Pharmacology Source Type: research

Voltammetric sensor based on cobalt-poly(methionine)-modified glassy carbon electrode for determination of estriol hormone in pharmaceuticals and urine
Publication date: Available online 5 April 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Eliziana S. Gomes, Fernando R.F. Leite, Bruno R.L. Ferraz, Henrique A.J.L. Mourão, Andréa R. MalaguttiAbstractA voltammetric sensor based on the electropolymerization of cobalt-poly(methionine) (Co-poly(Met)) on a glassy carbon electrode (GCE) was developed and applied for the determination of estriol by differential pulse voltammetry (DPV) for the first time. The electrochemical properties of the Co-poly(Met)/GCE were analysed by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Scannin...
Source: Journal of Pharmaceutical Analysis - April 5, 2019 Category: Drugs & Pharmacology Source Type: research

Highly sensitive simultaneous electrochemical determination of myricetin and rutin via solid phase extraction on a ternary Pt@r-GO@MWCNTs nanocomposite
Publication date: Available online 22 March 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Satar Tursynbolat, Yrysgul Bakytkarim, Jianzhi Huang, Lishi WangAbstractThe simultaneous electrochemical determination of myricetin and rutin remains a challenge due to their indistinguishable potentials. To solve this problem, we constructed a ternary platinum nanoparticle, reduced graphene oxide, multi-walled carbon nanotubes (Pt@r-GO@MWCNTs) nanocomposite via a facile one-pot synthetic method. Under the optimized conditions, the ternary Pt@r-GO@MWCNTs nanocomposite exhibited good electrocatalytic activity toward myriceti...
Source: Journal of Pharmaceutical Analysis - March 23, 2019 Category: Drugs & Pharmacology Source Type: research

Primmorph extracts and mesohyls of marine sponges inhibit proliferation and migration of hepatocellular carcinoma cells in vitro
Publication date: Available online 22 March 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Hanaa Rady, Sohair Salem, Mohamed Ez El-ArabAbstractCancer recurrence and severe side effects of currently being used chemotherapeutic agents reduce their clinical efficacy. Thus, there is a constant need to develop alternative anticancer drugs. Sustainable supply is an important challenge facing marine-based drug discovery. Primmorph, a 3D cell culture system, could provide a sustainable source to produce metabolites for anticancer drugs from marine sponges. In the present work, the anticancer activity of primmorph extract...
Source: Journal of Pharmaceutical Analysis - March 23, 2019 Category: Drugs & Pharmacology Source Type: research

Interaction of repaglinide with bovine serum albumin: Spectroscopic and molecular docking approaches
Publication date: Available online 16 March 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Suma K. Pawar, J. SeetharamappaAbstractRepaglinide (RPG) regulates the amount of glucose by stimulating the pancreas to release insulin in the blood. In view of its biological importance, we have examined the interaction between RPG and a model protein, bovine serum albumin (BSA) employing various spectroscopic, electrochemical and molecular docking methods. Fluorescence spectra of BSA were recorded in the presence and absence of RPG in phosphate buffer of pH 7.4. Fluorescence intensity of BSA was decreased upon the additio...
Source: Journal of Pharmaceutical Analysis - March 17, 2019 Category: Drugs & Pharmacology Source Type: research

Application of microfluidic chip technology in pharmaceutical analysis: A review
Publication date: Available online 6 December 2018Source: Journal of Pharmaceutical AnalysisAuthor(s): Ping Cui, Sicen WangAbstractThe development of pharmaceutical analytical methods represents one of the most significant aspects of drug development. Recent advances in microfabrication and microfluidics could provide new approaches for drug analysis, including drug screening, active testing and the study of metabolism. Microfluidic chip technologies, such as lab-on-a-chip technology, three-dimensional (3D) cell culture, organs-on-chip and droplet techniques, have all been developed rapidly. Microfluidic chips coupled with...
Source: Journal of Pharmaceutical Analysis - March 16, 2019 Category: Drugs & Pharmacology Source Type: research

Anticancer potential of metabolic compounds from marine actinomycetes isolated from Lagos Lagoon sediment
This study is the first to reveal the anticancer potential of actinomycetes from Lagos Lagoon, which could be exploited for therapeutic purposes. (Source: Journal of Pharmaceutical Analysis)
Source: Journal of Pharmaceutical Analysis - March 16, 2019 Category: Drugs & Pharmacology Source Type: research

Preparation of polypyrrole/nanosilica composite for solid-phase microextraction of bisphenol and phthalates migrated from containers to eye drops and injection solutions
Publication date: Available online 15 March 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Mehdi Ansari Dogaheh, Mansoureh BehzadiAbstractThis paper describes the electrodeposition of polyphosphate-doped polypyrrole/nanosilica nanocomposite coating on steel wire for direct solid-phase microextraction of bisphenol A and five phthalates. Influencing parameters on the extraction efficiency and morphology of the nanocomposite such as deposition potential, concentration of pyrrole and polyphosphate, deposition time and the nanosilica amount were optimized. Under the optimized conditions, characterization of the nanoco...
Source: Journal of Pharmaceutical Analysis - March 16, 2019 Category: Drugs & Pharmacology Source Type: research