UvrA and UvrC subunits of the Mycobacterium  tuberculosis UvrABC excinuclease interact independently of UvrB and DNA

The UvrABC excinuclease plays a vital role in bacterial nucleotide excision repair. While UvrA and UvrB subunits associate to form a UvrA2B2 complex, interaction between UvrA and UvrC has not been demonstrated or quantified in any bacterial species. Here, usingMycobacterium  tuberculosis UvrA (MtUvrA), UvrB (MtUvrB) and UvrC (MtUvrC) subunits, we show that MtUvrA binds to MtUvrB and equally well to MtUvrC with submicromolar affinity. Furthermore, MtUvrA forms a complex with MtUvrC bothin  vivo andin  vitro, independently of DNA and UvrB. Collectively, these findings reveal new insights into the pairwise relationships between the subunits of the UvrABC incision complex.
Source: FEBS Letters - Category: Biochemistry Authors: Tags: Research Article Source Type: research