Betamethasone Treatment for Atopic Dermatitis in Gut Microbiota Transplanted Mice.

Betamethasone Treatment for Atopic Dermatitis in Gut Microbiota Transplanted Mice. Comp Med. 2019 Nov 19;: Authors: Debes KP, Evdina NA, Laigaard A, Larsen JM, Zachariassen LF, Hansen CHF, Hansen AK Abstract Gut microbiota composition correlates strongly with essential disease parameters in the oxazolone-induced mouse model for atopic dermatitis. The phenotype of this model can be transferred to germ-free mice with a gut microbiota transplant to achieve high and low responding mice. Therefore, the production of high responding mice through gut microbiota transplantation may be seen as a tool to reduce group sizes or increase power in intervention studies by increasing effect size. We sought to determine whether high responding mice respond to a common treatment in the same way as low responding mice. We hypothesized that while high responding mice would exhibit a higher clinical score than low responding mice beforetreatment, the clinical parameters would be similar in both groups after betamethasone treatment. Dermatitis was induced with oxazolone in barrier bred Swiss Webster mice, and a high responding and a low responding donor was selected based upon clinical and pathologic scores, as confirmed by monitoring a range of ear tissue cytokines. Feces from these donors were transplanted to pregnant germ-free Swiss Webster dams, and subsequently to their offspring. Although the overall effect of betamethasone on the clinical dermatiti...
Source: Comparative Medicine - Category: Zoology Authors: Tags: Comp Med Source Type: research