Serinibacter arcticus sp. nov., isolated from a thawing ancient ice wedge.

Serinibacter arcticus sp. nov., isolated from a thawing ancient ice wedge. Int J Syst Evol Microbiol. 2019 Nov 15;: Authors: Filippova SN, Surgucheva NA, Detkova EN, Rakitin AL, Beletsky AV, Grouzdev DS, Kolganova TV, Mulyukin AL Abstract A novel actinobacterium, strain K3-2T, was isolated in pure culture from a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia). Colonies of strain K3-2T were yellowish orange; cells had the fine structure typical of Gram-positive bacteria, were non-motile short rods and were non-spore-forming. Strain K3-2T was mesophilic (optimum growth at 28 °С), but capable of growing at 4 °С. The cell-wall peptidoglycan of strain K3-2T contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine. The polar lipids were phosphatidylglycerol, lysophosphatidylserine, three unidentified phospholipids and glycolipids. The major fatty acids were anteiso-C15 : 0 and C16 : 0. The only menaquinone detected was MK-8(H4). 16S rRNA gene analysis indicated that strain K3-2T belongs to the genus Serinibacter. The closest taxonomically described relatives were Serinibacter salmoneus Kis4-28T and Serinibacter tropicus PS-14-7T, with 97.20 and 97.20 % 16 s rRNA gene sequence similarity, respectively. The average nucleotide identity value of the whole genome sequence between strain K3-2T and S. salmoneus Kis4-28T was 78.9 %. DNA-DNA relatedness values be...
Source: International Journal of Systematic and Evolutionary Microbiology - Category: Microbiology Authors: Tags: Int J Syst Evol Microbiol Source Type: research