A real-time PCR for rapid identification of Salmonella enterica Gaminara serovar

This report outlines the development of a rapid multiplex real-time PCR procedure that facilitates the identification of Salmonella serogroup I and the serovars of the group. Using Salmonella Gaminara serovar (O16:d:1,7) as an example, first the gene(s) responsible for expression of the somatic O antigen, O16, and the nucleotide sequence of the variable-region of genes encoding the flagellar phase-1 (d) and phase-2 (1,7) antigens were identified. Then, a multiplex real-time PCR was designed that incorporated primers and probes specific for the three target genes and confirmed the specificity. The assay had 100% inclusivity for all three gene targets, detecting 2 genomic DNA copies of O16 and 1,7 gene targets and 10 copies of d gene target.Importance: Rapid molecular methods to identify Salmonella serovars should increase the precision of routine surveillance of clinically important serovars and promote public health.
Source: Journal of Microbiological Methods - Category: Microbiology Source Type: research

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Despite an outbreak of vegetable-associated Salmonella poisoning linked to products sold at Massachusetts restaurants that has affected 90 Massachusetts residents and resulted in three deaths, the Massachusetts Department of Public Health is refusing to release any information on the restaurants at which the patients ate or the exact products they consumed. The Department has revealed only that the outbreak is associated with " vegetable products " and that some of the patients reported having eaten vegetables at restaurants in the Commonwealth.The Department of Public Health is not even telling the restaurant ow...
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Source: Food Research International - Category: Food Science Source Type: research
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Source: Food Research International - Category: Food Science Source Type: research
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Source: Food Research International - Category: Food Science Source Type: research
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Source: Food Research International - Category: Food Science Source Type: research
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PMID: 31751325 [PubMed - in process]
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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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