A high content screening assay for identifying inhibitors against active and dormant state intracellular Mycobacterium tuberculosis
Publication date: Available online 12 August 2019Source: Journal of Microbiological MethodsAuthor(s): Amar Yeware, Sonia Agrawal, Dhiman SarkarAbstractThe antitubercular drug development pipeline could start with an in vitro investigation of several compounds to examine their effect on active and dormant state Mycobacterium tuberculosis (Mtb). However, in vitro screening of dormant state bacilli cannot provide enough information on the simultaneous effect of a compound on the host. Therefore, we developed a live cell fluorescence based screening protocol by utilizing the high content system for determining the effect of in...
Source: Journal of Microbiological Methods - August 13, 2019 Category: Microbiology Source Type: research

Simple, fast and accurate method for the determination of glycogen in the model unicellular cyanobacterium Synechocystis sp. PCC 6803
The objective of this study is to optimize the methodology for the determination of glycogen in cyanobacteria, as the interest in cyanobacterial glycogen has increased in recent years due to the biotechnological application of these microorganisms. In the present work, the methodology reported for the quantification of glycogen in cyanobacteria has been reviewed and an extensive empirical analysis has been performed showing how this methodology can be optimized significantly to reduce time and improve reliability and reproducibility. Based on these results, a simple and fast protocol for quantification of glycogen in the m...
Source: Journal of Microbiological Methods - August 9, 2019 Category: Microbiology Source Type: research

Bacterial identification using an Absciex 5800 TOF/TOF MALDI research instrument and an external database
Publication date: Available online 7 August 2019Source: Journal of Microbiological MethodsAuthor(s): Charlotte A. Huber, Valentin Pflüger, Sarah Reed, Kyra Cottrell, Hanna E. Sidjabat, Ama Ranasinghe, Hosam Zowawi, Patrick Harris, David L. PatersonAbstractIn our current study we were identifying 26 bacterial isolates using an Absciex 5800 TOF/TOF MALDI instrument and an external database. The results were compared with the results of a Vitek® MS system and in case of discrepancies at the species level 16s rRNA sequencing was performed for further verification. (Source: Journal of Microbiological Methods)
Source: Journal of Microbiological Methods - August 9, 2019 Category: Microbiology Source Type: research

Evaluation of RNA degradation in pure culture and field Microcystis samples preserved with various treatments
In this study, four different treatments (−80 °C freezer, −196 °C liquid nitrogen, 4 °C or 25 °C preservation after adding RNA protective fluid) were employed to preserve RNA in pure culture and field Microcystis samples, and RNA degradation in these treatments were systematically evaluated. Results showed liquid nitrogen was the most effective treatment to preserve RNA in pure culture and field Microcystis samples. RNA preservation using RNA protective fluid was temperature dependent. Low temperature (4 °C) could effectively slow down RNA degradation within a short time (1–7...
Source: Journal of Microbiological Methods - August 6, 2019 Category: Microbiology Source Type: research

The prevalence of Aspergillus fumigatus in early cystic fibrosis disease is underestimated by culture-based diagnostic methods
Publication date: Available online 3 August 2019Source: Journal of Microbiological MethodsAuthor(s): Emma Reece, Siobhán McClean, Peter Greally, Julie RenwickAbstractAspergillus fumigatus is the most common fungus infecting/colonising people with cystic fibrosis (CF) and can negatively impact clinical status. Diagnostic laboratories rely on culture to detect A. fumigatus which is known to be less sensitive than molecular approaches. Therefore, A. fumigatus colonisation in the CF population may be underestimated.Sputum (n = 60) from 25 children with CF were collected and A. fumigatus was detected using routine cu...
Source: Journal of Microbiological Methods - August 4, 2019 Category: Microbiology Source Type: research

Fast fluorometric enumeration of E. coli using passive chip
In this report, a passive microfluidic chip design was developed for fast and sensitive fluorometric determination of Escherichia coli (E. coli) based on sandwich immunoassay. Initially, magnetic nanoparticles (MNPs) and chitosan modified mercaptopropionic acid capped cadmium telluride (CdTe) quantum dots (QDs) were functionalized with E.coli specific antibody to form a sandwich immunoassay with the E. coli. The magnetic separation and preconcentration of the E.coli from the sample solution was performed in the vial. Conjugation of QDs to the magnetically captured E. coli and washing were performed using a passive type of ...
Source: Journal of Microbiological Methods - August 3, 2019 Category: Microbiology Source Type: research

Automated image analysis with ImageJ of yeast colony forming units from cannabis flowers
Publication date: Available online 2 August 2019Source: Journal of Microbiological MethodsAuthor(s): Nicholas Stolze, Carly Bader, Christopher Henning, Jared Mastin, Andrea E. Holmes, Arin L. SutliefAbstractCurrently, in the state of Colorado and all other states within the United States of America with legalized marijuana programs, testing is required for bacteria, yeast, and mold on marijuana products. The Code of Colorado Regulations, 1 CCR 212–1, considers a passing result when a 1 g sample contains
Source: Journal of Microbiological Methods - August 3, 2019 Category: Microbiology Source Type: research

Enumeration of industrial Bacillus assemblages in commercial products with customized plate-counting assays
Publication date: Available online 2 August 2019Source: Journal of Microbiological MethodsAuthor(s): John P. Gorsuch, Zachary Jones, Daniel Le Saint, Christopher L. KittsAbstractThe aerobic plate count assay remains among the most widespread methods for enumerating industrial Bacillus assemblages, as growth-independent methods are either cost prohibitive or unavailable in some areas. However, the standard plating assays used to verify the CFU count of Bacillus-based products are not tailored to Bacillus species and thus may not produce the most accurate possible estimations. Standard plating assays assume that established ...
Source: Journal of Microbiological Methods - August 3, 2019 Category: Microbiology Source Type: research

Sterilization performance comparison between an autosampler-ready microporous filter vial and a syringe-based filter
Publication date: Available online 26 July 2019Source: Journal of Microbiological MethodsAuthor(s): ThomasD. Horvath, Sibel Ak, Sigmund J. Haidacher, Kathleen Hoch, Tor C. Savidge, Anthony M. HaagAbstractHerein we report that the Thomson Standard and eXtreme/FV® filter vials (0.2 μm polyvinylidene difluoride filter membrane) are as effective as gold standard microporous membrane-based syringe filters at removing bacteria, such as Klebsiella pneumonia, from media samples produced in the microbiological laboratory. (Source: Journal of Microbiological Methods)
Source: Journal of Microbiological Methods - July 27, 2019 Category: Microbiology Source Type: research

Comparison of heating block and water bath methods to determine heat resistance in Shiga-toxin producing Escherichia coli with and without the locus of heat resistance
This study found variability in the time required for tubes of media in heating block wells to reach 60 °C, resulting in significant effects on heat resistance measurements. To determine the extent that methodology changed heat resistance measurements, we compared the heat resistance of Shiga-toxin producing Escherichia coli (STEC) strains with and without the locus of heat resistance (LHR) using both heating block and water bath methods. A total of 34 strains of STEC were used along with a generic E. coli which has been identified as heat-resistant and used as a positive control. The E. coli strains were incubated i...
Source: Journal of Microbiological Methods - July 27, 2019 Category: Microbiology Source Type: research

A 13-Plex PCR for high-resolution melting-based screening of clinically important Staphylococcus species in animals
Publication date: Available online 23 July 2019Source: Journal of Microbiological MethodsAuthor(s): Zafer Ata, Esra BuyukcangazAbstractA single-tube multiplex real-time PCR targeting the nuclease (nuc) gene and subsequent high-resolution melting analysis (HRMA) were used to identify 13 different Staphylococcus species. The nuc gene was targeted due to its low intraspecies variation and the greater interspecies variation than the 16S rRNA gene in Staphylococcus. We used HRMA software that can store and compare HRMA profiles from different runs as long as the runs contain the same reference reaction. Thus, we reduced the 14 ...
Source: Journal of Microbiological Methods - July 24, 2019 Category: Microbiology Source Type: research

One piece biopsy for both rapid urease test and cultivation of Helicobacter pylori
ConclusionThe researchers attempted to use one biopsy specimen for both rapid urease and culture tests. This method causes fewer injuries of gastric tissue and allows antimicrobial susceptibility testing and characterization in detail of the isolated organism. (Source: Journal of Microbiological Methods)
Source: Journal of Microbiological Methods - July 24, 2019 Category: Microbiology Source Type: research

A novel isothermal amplification-based method for detection of Corynebacterium striatum
In this study, a C. striatum-multiple cross displacement amplification (MCDA) with visual detection reagent (VR) assay (C. striatum-MCDA-VR), which was a novel isothermal amplification-based method, was established to detect the species-specific ftr1 gene of C. striatum. Amplification was performed at a constant temperature (68 °C) for only 40 min, and the reaction results could be easily elucidated by observation of reaction mixture color when employing the VR. The limit of detection of this method was 10 fg of pure C. striatum DNA. No cross-reaction was observed with non-C. striatum strains. In testing of clini...
Source: Journal of Microbiological Methods - July 24, 2019 Category: Microbiology Source Type: research

Establishment of loop-mediated isothermal amplification for rapid and convenient detection of Mycobacterium marinum complex
Publication date: Available online 18 July 2019Source: Journal of Microbiological MethodsAuthor(s): Ming-An Tsai, Pei-Chi Wang, Shiomi Yoshida, Akio Aono, Satoshi Mitarai, Takayuki Wada, Shih-Chu ChenAbstractMycobacterium marinum is a zoonotic pathogen that can cause dermatological infection mainly from contaminated water or fish. Some well-known genetically similar species and subspecies are M. lifrandii and M. pseudoshottsii from amphibians and fish in aquaculture, and M. ulcerans, a causative agent of a neglected tropical disease (NTD), Buruli ulcer. They are believed to survive in water as their major niche, which migh...
Source: Journal of Microbiological Methods - July 19, 2019 Category: Microbiology Source Type: research

The investigation of media components for optimal metabolite production of Aspergillus terreus ATCC 20542
ConclusionThe findings from these investigations could be used for future cultivation of A. terreus in the production of desired metabolites. (Source: Journal of Microbiological Methods)
Source: Journal of Microbiological Methods - July 19, 2019 Category: Microbiology Source Type: research

Use of synthesized double-stranded gene fragments as qPCR standards for the quantification of antibiotic resistance genes
Publication date: Available online 17 July 2019Source: Journal of Microbiological MethodsAuthor(s): Like Xu, Hannah Chen, Melisa Canales, Lena CiricAbstractPollution of various environmental matrices by antibiotic resistance genes (ARGs) has become a growing threat to human health. For the quantitative analysis of the presence of ARGs, there is a need for sensitive and robust qPCR assays which can detect various genes from different types of DNA extracts. Fourteen ARGs were selected as target genes in this study including: blaTEM, blaOXA-1 and blaCTX-M coded for resistance to β-lactams; ermB coded for macrolides; tetA...
Source: Journal of Microbiological Methods - July 18, 2019 Category: Microbiology Source Type: research

Storage and handling of human faecal samples affect the gut microbiome composition: A feasibility study
Publication date: Available online 11 July 2019Source: Journal of Microbiological MethodsAuthor(s): Alan C. Ezzy, Amanda D. Hagstrom, Chris George, Adam S. Hamlin, Lily Pereg, Aron J. Murphy, Gal WinterAbstractHuman gut microbiome analysis through faecal sampling typically involves five stages: sample collection, storage, DNA extraction, next generation sequencing and bioinformatics analysis. Of these, the first three are considered irreversible. This feasibility study describes an assessment of methodologies used for faecal DNA extraction and sample handling, using the parameters DNA yield, purity and resultant microbial ...
Source: Journal of Microbiological Methods - July 12, 2019 Category: Microbiology Source Type: research

A thermal ink-jet printing approach for evaluating susceptibility of bacteria to antibiotics
Publication date: Available online 10 July 2019Source: Journal of Microbiological MethodsAuthor(s): Cornelius C. Dodoo, Mustafa Alomari, Abdul W. Basit, Paul Stapleton, Simon GaisfordAbstractAn inexpensive method for determining minimum inhibitory concentrations (MIC) using ink-jet printing to deposit drug solutions and bacterial suspensions onto agar was developed. Substrate concentrations were varied using a “Y-value”, whereby a series of rectangles with the same width and colour but different heights were printed within a fixed unit area. Prior to MIC determination, the printer cartridges used were calibrate...
Source: Journal of Microbiological Methods - July 12, 2019 Category: Microbiology Source Type: research

Comparing methods of genetic manipulation in Bacillus subtilis for expression of recombinant enzyme: Replicative or integrative (CRISPR-Cas9) plasmid?
Publication date: Available online 8 July 2019Source: Journal of Microbiological MethodsAuthor(s): Kamila Oliveira Santos, João Costa-Filho, Kérolin Luana Spagnol, Luis Fernando MarinsAbstractThe present study evaluated the stability of Bacillus subtilis strains transformed with a replicative or integrative plasmid (via CRISPR-Cas9) to express a recombinant phytase. Both transformation methods did not affect the growth of B. subtilis, but the stability of the construct and the enzymatic activity was reduced in the strain transformed with the replicative plasmid. (Source: Journal of Microbiological Methods)
Source: Journal of Microbiological Methods - July 9, 2019 Category: Microbiology Source Type: research

Editorial Board
Publication date: August 2019Source: Journal of Microbiological Methods, Volume 163Author(s): (Source: Journal of Microbiological Methods)
Source: Journal of Microbiological Methods - July 8, 2019 Category: Microbiology Source Type: research

Zygosaccharomyces bailii and Z. rouxii induced ethanol formation in apple juice supplemented with different natural preservatives: A response surface methodology approach
In this study, ethanol produced by osmophilic yeasts, Zygosaccharomyces bailii and Z. rouxii, in apple juice preserved with mint essential oil (MEO), carvacrol and natamycin instead of synthetic preservatives was modeled. Some processing parameters such as sodium benzoate (SB, 0–0.1%) used as a positive control, storage temperature (4–20 °C) and storage time (1–41 days) were selected in the study. Box-Behnken design in response surface methodology was used to evaluate the effects of processing parameters on ethanol levels of apple juice and three models were created for three preservatives for eac...
Source: Journal of Microbiological Methods - July 2, 2019 Category: Microbiology Source Type: research

A facile one pot synthesis of novel pyrimidine derivatives of 1,5-benzodiazepines via domino reaction and their antibacterial evaluation
Publication date: August 2019Source: Journal of Microbiological Methods, Volume 163Author(s): Apoorva Misra, Sonika Jain, Dharma Kishore, Vivek Dave, Kakarla Raghava Reddy, Veera Sadhu, Jaya Dwivedi, Swapnil SharmaAbstractA new series of pyrimidine (8, 14, 18 and 23) embellished analogues of 1,5-benzodiazepines were synthesized by the one-pot domino approach using the catalyst DABCO (1,4-diazabicyclo[2.2.2]octane). For each compound synthesized, anti-microbial efficacy was determined using broth microdilution assay and half maximal inhibitory concentration (IC50). Furthermore, FESEM (Field emission scanning electron micros...
Source: Journal of Microbiological Methods - June 29, 2019 Category: Microbiology Source Type: research

Validation of reference genes for quantitative gene expression analysis in Auricularia cornea
In this study, nine candidate genes, including tubulin alpha-1A chain (TUBA1A), β-tubulin (Btu), phosphoglucomutase (Pgm), actin 1 (Act1), protein phosphatase 2A regulatory subunit (PP2A), polyubiquitin (UBQ), glyceraldehyde-3-phosphate dehydrogenase (Gapdh), 18S ribosomal protein (18S) and 28S ribosomal protein (28S), were evaluated among different strains and developmental stages. Four algorithms (i.e., geNorm, NormFinder, BestKeeper and RefFinder) were used to analyze candidate genes. The results revealed that UBQ was the most stable reference gene, while 18S was the least stable. Despite these results, the candida...
Source: Journal of Microbiological Methods - June 26, 2019 Category: Microbiology Source Type: research

Micro-staining microbes: An alternative to traditional staining of microbiological specimens using microliter volumes of reagents
Publication date: Available online 24 June 2019Source: Journal of Microbiological MethodsAuthor(s): Nicole Siguenza, Aditya Jangid, E. Brandon Strong, John Merriam, Andres W. Martinez, Nathaniel W. MartinezAbstractMicrobial staining techniques are widely employed in clinical and academic laboratories for classifying and identifying microorganisms derived from clinical, food and environmental samples. Staining allows for the rapid visualization and determination of many morphological characteristics of microorganisms, used for their identification and classification. Over the past century, staining techniques such as the Gr...
Source: Journal of Microbiological Methods - June 26, 2019 Category: Microbiology Source Type: research

Zygosaccharomyces bailii and Z. rouxii: Induced ethanol formation in apple juice supplemented with different natural preservatives: A response surface methodology approach
In this study, ethanol produced by osmophilic yeasts, Zygosaccharomyces bailii and Z. rouxii, in apple juice preserved with mint essential oil (MEO), carvacrol and natamycin instead of synthetic preservatives was modeled. Some processing parameters such as sodium benzoate (SB, 0–0.1%) which is a positive control, storage temperature (4–20 °C) and storage time (1–41 days) were selected in the study. Box-Behnken design in response surface methodology was used to evaluate the effects of processing parameters on ethanol levels of apple juice and three models were created for three preservatives for ea...
Source: Journal of Microbiological Methods - June 26, 2019 Category: Microbiology Source Type: research

Evaluation of Q Gene Mycobacteria: A novel and easy nucleic acid chromatography method for mycobacterial species identification
ConclusionsQ Gene Mycobacteria was able to identify frequently isolated mycobacterial species accurately and easily. Therefore, Q Gene Mycobacteria could be a useful tool for the identification of specific mycobacteria in clinical laboratories. (Source: Journal of Microbiological Methods)
Source: Journal of Microbiological Methods - June 20, 2019 Category: Microbiology Source Type: research

Efficient genome editing in Aspergillus niger with an improved recyclable CRISPR-HDR toolbox and its application in introducing multiple copies of heterologous genes
Publication date: Available online 18 June 2019Source: Journal of Microbiological MethodsAuthor(s): Hongzhi Dong, Junwei Zheng, Dou Yu, Bin Wang, Li PanAbstractAspergillus niger is an important industrial producer of enzymes due to its high capacity for producing exocellular secretory proteins. The CRISPR/Cas9 system has been developed as a genetic manipulation tool in A. niger. However, only the basic functions of the CRISPR/Cas9 system, such as codon optimization of Cas9 nucleases and promoter screening of guide RNA (gRNA) expression, have been developed in A. niger. The CRISPR/Cas9 system for manipulating large genomic ...
Source: Journal of Microbiological Methods - June 20, 2019 Category: Microbiology Source Type: research

Algae-based metallic nanoparticles: Synthesis, characterization and applications
Publication date: Available online 17 June 2019Source: Journal of Microbiological MethodsAuthor(s): Prerna Khanna, Amrit Kaur, Dinesh GoyalAbstractNanomaterials (NMs) tailored via conventional physicochemical routes play havoc with the environment that has led to the evolution of competent green routes for the actualization of a circular economy on an industrial-scale. Algae belonging to the class Cyanophyceae, Chlorophyceae, Phaeophyceae and Rhodophyceae have been harnessed as nano-machineries through intracellular and extracellular synthesis of gold (Au), silver (Ag) and several other metallic nanoparticles. Algae are an...
Source: Journal of Microbiological Methods - June 18, 2019 Category: Microbiology Source Type: research

Development and evaluation of the method for detecting metallo-carbapenemases among carbapenemase-producing Enterobacteriaceae
Publication date: Available online 13 June 2019Source: Journal of Microbiological MethodsAuthor(s): Quanfeng Liao, Yun Xie, Chengcheng Wang, Zhiyong Zong, Siying Wu, Ya Liu, Weili Zhang, Yi Xie, Yuling Xiao, Mei KangAbstractA simple EDTA synergistic carbapenem inactivation method (esCIM) based on the simplified carbapenem inactivation method (sCIM) and EDTA synergistic carbapenem inactivation test (eCIM) detected the levels of metallo-β-lactamases (MBLs) carbapenemase. The esCIM method uses EDTA-impregnated antibiotic disk to detect carbapenemase-producing Enterobacteriaceae (CPE) directly instead of inculating the di...
Source: Journal of Microbiological Methods - June 14, 2019 Category: Microbiology Source Type: research

Development of a loop-mediated isothermal amplification assay for rapid Helicobacter pylori detection
Publication date: Available online 11 June 2019Source: Journal of Microbiological MethodsAuthor(s): Saori Horiuchi, Ryuichi Nakano, Akiyo Nakano, Naokuni Hishiya, Kenji Uno, Yuki Suzuki, Ayako Tanouchi, Naoki Kakuta, Takashi Masui, Noriko Jojima, Hisakazu YanoAbstractInfection with cagA-positive Helicobacter pylori is associated with gastric cancer. Molecular techniques are vital for accurate H. pylori diagnosis. We developed a loop-mediated isothermal amplification (LAMP) for detecting the H. pylori cagA gene and evaluated its use for clinical diagnosis. A LAMP primer set was designed to recognize the homologous regions o...
Source: Journal of Microbiological Methods - June 12, 2019 Category: Microbiology Source Type: research

Antibacterial properties of novel pyrimidine compounds
Publication date: Available online 10 June 2019Source: Journal of Microbiological MethodsAuthor(s): A. Misra, S. Jain, D. Kishore, V. Dave, K.R. Reddy, Veera Sadhu, J. Dwivedi, S. SharmaAbstractA new series of pyrimidine (8, 14, 18 and 23) embellished analogues of 1,5-benzodiazepines were synthesized by the one-pot domino approach using the catalyst DABCO (1,4-diazabicyclo[2.2.2]octane). For each compound synthesized, anti-microbial efficacy was determined using broth microdilution assay and half maximal inhibitory concentration (IC50). Furthermore, FESEM (Field emission scanning electron microscope) studies were also carr...
Source: Journal of Microbiological Methods - June 12, 2019 Category: Microbiology Source Type: research

Consistent production of chlorine-stressed bacteria from non-chlorinated secondary sewage effluents for use in the U.S. Environmental Protection Agency alternate test procedure protocol
We describe a starting point for generating chlorine-stressed samples from secondary effluent to evaluate detection technologies to demonstrate comparability to EPA reference methods. (Source: Journal of Microbiological Methods)
Source: Journal of Microbiological Methods - June 8, 2019 Category: Microbiology Source Type: research

A review on various maleic anhydride antimicrobial polymers
This article also addresses the applications of antimicrobial polymers with maleic anhydride in numerous sectors.Graphical abstractFigure: Various maleic anhydride polymers exhibiting antimicrobial activity. (Source: Journal of Microbiological Methods)
Source: Journal of Microbiological Methods - June 8, 2019 Category: Microbiology Source Type: research

Evaluation of modified Zobell marine agar for differential isolation of histamine-forming bacteria from fresh fish
Publication date: Available online 5 June 2019Source: Journal of Microbiological MethodsAuthor(s): Suma Devivilla, Jerusha Stephen, Manjusha Lekshmi, Sanath H. Kumar, Binaya Bhusan Nayak (Source: Journal of Microbiological Methods)
Source: Journal of Microbiological Methods - June 6, 2019 Category: Microbiology Source Type: research

Editorial Board
Publication date: July 2019Source: Journal of Microbiological Methods, Volume 162Author(s): (Source: Journal of Microbiological Methods)
Source: Journal of Microbiological Methods - June 5, 2019 Category: Microbiology Source Type: research

A novel microbiological medium for the growth of periodontitis associated pathogens
Publication date: Available online 30 May 2019Source: Journal of Microbiological MethodsAuthor(s): Niall A. Hickey, Kathryn A. Whitehead, Liliana Shalamanova, Jonathan A. Butler, Rebecca L. TaylorAbstractA novel microbiological medium designed to be more representative of gingival crevicular fluid. Chosen representative periodontal microorganisms showed good growth with minimal effect on human cell viability. This will enable more comparisons between different periodontitis associated organisms and their potential role in host health and systemic disease. (Source: Journal of Microbiological Methods)
Source: Journal of Microbiological Methods - May 30, 2019 Category: Microbiology Source Type: research

Optimisation of a droplet digital PCR for strain specific quantification of a probiotic Bifidobacterium animalis strain in poultry feed
Publication date: Available online 30 May 2019Source: Journal of Microbiological MethodsAuthor(s): Sergi Raurich, Barbara Weber, Viviana Klose, Michaela Mohnl, Daniel Petri, Silvia Fibi-SmetanaAbstractThe use of probiotics in animal nutrition to provide health benefits is widely accepted. Bifidobacterium animalis (BAN) is an example of a commonly used beneficial strain. BAN is applied in a multi-strain feed additive for poultry. As part of the increased demand for tracking and tracing of feed additives within modern quality management, it is crucial to determine the quantity of the active strain after mixing the probiotic ...
Source: Journal of Microbiological Methods - May 30, 2019 Category: Microbiology Source Type: research

Development of the Com1 synthetic peptide-based Latex Agglutination Test (LAT) and its comparative evaluation with commercial indirect-ELISA for sero-screening of coxiellosis in cattle
Publication date: Available online 27 May 2019Source: Journal of Microbiological MethodsAuthor(s): Manesh Kumar, Satyaveer Singh Malik, Jess Vergis, Sunitha Ramanjeneya, Radhakrishna Sahu, Richa Pathak, Jay Prakash Yadav, Pankaj Dhaka, Sukhadeo B. Barbuddhe, Deepak B. RawoolAbstractA novel Com1 synthetic peptide-based latex agglutination test (LAT) was developed and evaluated against commercial ELISA kit for sero-screening of coxiellosis in cattle. The developed test is economical, has field applicability and can serve as an important rapid tool for sero-screening of coxiellosis in cattle. (Source: Journal of Microbiological Methods)
Source: Journal of Microbiological Methods - May 29, 2019 Category: Microbiology Source Type: research

Cyanobacteria as a bioreactor for synthesis of silver nanoparticles-an effect of different reaction conditions on the size of nanoparticles and their dye decolorization ability
Publication date: Available online 24 May 2019Source: Journal of Microbiological MethodsAuthor(s): Shaheen Husain, Sumbul Afreen, Hemlata, Durdana Yasin, Bushra Afzal, Tasneem FatmaAbstractThe green synthesis of metallic nanoparticles has paved the way for improving and protecting the environment by decreasing the use of toxic chemicals and eliminating biological risks in biomedical applications. Biological synthesis of metal nanoparticles is gaining more importance due to simplicity, rapid rate of synthesis and eco-friendliness. In the present investigation cyanobacterial (Microchaete NCCU-342) cell free aqueous extract h...
Source: Journal of Microbiological Methods - May 24, 2019 Category: Microbiology Source Type: research

Comparison of real-time PCR and droplet digital PCR for the detection of Xylella fastidiosa in plants
In this study, a real-time PCR protocol, already used for Xf detection in the framework of official surveys in the European Union, was transferred and optimized for Xf detection using ddPCR. This new assay was evaluated and compared to the initial real-time PCR on five plant matrices artificially inoculated and on naturally infected plants. In our conditions, this new ddPCR enabled the detection of Xf on all artificially inoculated plant macerates with a similar limit of detection, or a slight benefit for Quercus ilex. Moreover, ddPCR improved diagnostic sensitivity as it enabled detection of Xf in samples of Polygala myrt...
Source: Journal of Microbiological Methods - May 22, 2019 Category: Microbiology Source Type: research

Editorial Board
Publication date: June 2019Source: Journal of Microbiological Methods, Volume 161Author(s): (Source: Journal of Microbiological Methods)
Source: Journal of Microbiological Methods - May 21, 2019 Category: Microbiology Source Type: research

Rapid antibiotic susceptibility testing by resazurin using thin film platinum as a bio-electrode
We present a rapid screening method to evaluate the response of bacteria to antibiotics based upon the electrochemical measurement of live bacterial cell metabolic activity using an electroactive redox dye, resazurin. A thin film of Pt deposited over a glass substrate using the direct current sputtering technique was used as a working bio-electrode for electrochemical readouts. X-ray diffraction and scanning electron microscopy was carried out to characterize the Pt thin film. We tested the efficacy of the method using two different strains, Klebsiella pneumoniae (ATCC-700603) and Escherichia coli (ATCC-25922), against amp...
Source: Journal of Microbiological Methods - May 17, 2019 Category: Microbiology Source Type: research

Detection of Enterocytozoon hepatopenaei using an invasive but non-lethal sampling method in shrimp (Penaeus vannamei)
In this study, we describe an invasive but non-lethal sampling methodology using a syringe to collect biopsy samples from the hepatopancreas (HP) of Penaeus vannamei to detect the microsporidian pathogen, Enterocytozoon hepatopenaei (EHP), by qPCR and transmission electron microscopy (TEM). EHP was detected in all the infected shrimp by qPCR. The shrimp infected by the microsporidian showed 65% survival at 7 days post-sampling. Transmission electron microscopic examination of the biopsy samples revealed numerous spores of the pathogen. The presence of EHP was further confirmed by histology and in situ hybridization from ...
Source: Journal of Microbiological Methods - May 17, 2019 Category: Microbiology Source Type: research

Pitfalls of molecular diagnostic testing for Coxiella burnetii DNA on throat swabs
ConclusionC. burnetii PCR on throat swabs is of low diagnostic value for Q fever pneumonia and was false positive in 15.3% of CAP patients without Q fever pneumonia. Recent consumption of C. burnetii-containing products can influence the outcome of C. burnetii PCR on throat swabs. Therefore, diagnosis of C. burnetii infection should be made in combination with serology or PCR performed on blood. (Source: Journal of Microbiological Methods)
Source: Journal of Microbiological Methods - May 16, 2019 Category: Microbiology Source Type: research

Precise, direct, and rapid detection of Shigella Spa gene by a novel unmodified AuNPs-based optical genosensing system
In conclusion, the described method may develop into a platform that could be utilized for detection of various bacterial species with high accuracy and prompt screening of samples.Graphical abstract (Source: Journal of Microbiological Methods)
Source: Journal of Microbiological Methods - May 16, 2019 Category: Microbiology Source Type: research

Development of a rapid viability polymerase chain reaction method for detection of Yersinia pestis
Publication date: Available online 13 May 2019Source: Journal of Microbiological MethodsAuthor(s): Staci R. Kane, Sanjiv R. Shah, Teneile M. AlfaroAbstractDue to the occurrence of natural plague outbreaks and its historical usage as a biological weapon, Yersinia pestis is considered one of the high-priority biological threat agents. It can remain viable in certain environments including water for>100 days. Because of its slow-growth characteristic, it usually takes three or more days to detect and confirm the identity of viable Y. pestis cells by PCR, serological, or biochemical assays when using the traditional micro...
Source: Journal of Microbiological Methods - May 15, 2019 Category: Microbiology Source Type: research

Customizable 3D printed diffusion chambers for studies of bacterial pathogen phenotypes in complex environments
Publication date: Available online 11 May 2019Source: Journal of Microbiological MethodsAuthor(s): Lyddia Wilson, Kanwal Mohammad Iqbal, Terrie Simmons-Ehrhardt, Massimo F. Bertino, Muhammed Raza Shah, Vamsi K. Yadavalli, Christopher J. EhrhardtAbstractGaps in our understanding of the natural ecology and survival mechanisms of pathogenic bacteria in complex microenvironments such as soil typically occur due to the difficulty in characterizing biochemical profiles and morphological characteristics as they exist in environmental samples. Conversely, accurate simulation of the abiotic and biotic chemistries of soil habitats w...
Source: Journal of Microbiological Methods - May 12, 2019 Category: Microbiology Source Type: research

Colorimetric microdilution assay: Validation of a standard method for determination of MIC, IC50%, and IC90% of antimicrobial compounds
Publication date: Available online 9 May 2019Source: Journal of Microbiological MethodsAuthor(s): Andressa Veiga, Maria da Graça T. Toledo, Luciane S. Rossa, Mariana Mengarda, Nayana C.F. Stofella, Laiane J. Oliveira, Alan G. Gonçalves, Fábio S. MurakamiAbstractThe emergence of multiresistant bacteria directly impacts on the search for new compounds with antimicrobial activity, and it is important the improvemed of new techniques are able to determine the minimum inhibitory concentration (MIC) of antimicrobial compounds. The microdilution technique is widely used for saving culture media, reagents and ...
Source: Journal of Microbiological Methods - May 10, 2019 Category: Microbiology Source Type: research

Encapsulation of proteins from Leishmania panamensis into PLGA particles by a single emulsion-solvent evaporation method
Publication date: Available online 10 May 2019Source: Journal of Microbiological MethodsAuthor(s): Juan David Ospina-Villa, Catalina Gómez-Hoyos, Robin Zuluaga-Gallego, Omar Triana-ChávezAbstractThe current therapy for the treatment of leishmaniasis is unsatisfactory because it has multiple side effects, and resistance has been reported among the parasites that cause these diseases. Numerous efforts have been made to develop new candidates for vaccines. In recent years, particles of biodegradable polymers have been proposed as vehicles to transport and protect antigens, proteins, drugs and vaccines. In this w...
Source: Journal of Microbiological Methods - May 10, 2019 Category: Microbiology Source Type: research

Comparison of biochemical and genotypic speciation methods for vancomycin-resistant enterococci isolated from urban wastewater treatment plants
Publication date: Available online 6 May 2019Source: Journal of Microbiological MethodsAuthor(s): Haley Sanderson, Rodrigo Ortega-Polo, Kevin McDermott, Rahat Zaheer, R. Stephen Brown, Anna Majury, Tim McAllister, Steven N. LissAbstractEnterococci species in wastewater including Enterococcus faecalis, Enterococcus faecium, Enterococcus casseliflavus and Enterococcus gallinarum isolates (n = 308) with low or high level vancomycin resistance were determined and compared using a phenotypic method (RapID™ STR system), 16S rRNA sequencing, and multi-locus (atpA, groESL, and pheS) sequence analysis (MLSA). Error rates ...
Source: Journal of Microbiological Methods - May 7, 2019 Category: Microbiology Source Type: research