GSE139909 Role of the LytSR two-component regulatory system in Staphylococcus lugdunensis biofilm formation and pathogenesis

Contributors : Sandrine Dahyot ; Virginie Oxaran ; Ma ïté Niepceron ; Eddy Dupart ; Stéphanie Legris ; Jennifer Didi ; Thomas Clamens ; Olivier Lesouhaitier ; Yasmine Zerdoumi ; Jean M Flaman ; Martine Pestel-CaronSeries Type : Expression profiling by arrayOrganism : Staphylococcus lugdunensisStaphylococcus lugdunensis is a coagulase negative Staphylococcus, recognized as a virulent pathogen. It is responsible for a wide variety of infections, some of which being associated with biofilm production, such as implanted medical devices infections or endocarditis. However, little is known about S. lugdunensis virulence regulation. Two-component regulatory systems (TCS) play critical roles in bacterial adaptation, survival and virulence. Among them, LytSR is widely conserved, but has variable roles in different organisms, all being connected to metabolism or cell death and lysis into biofilms. Therefore, we investigated here the functions of LytSR in S. lugdunensis pathogenesis. Deletion of lytSR in S. lugdunensis DSM 4804 strain did not alter neither the susceptibility to Triton X-100 induced autolysis nor the death induced by antibiotics targeting cell wall synthesis. Interestingly, ΔlytSR biofilm was characterized by a lower biomass, a lack of tower structures and a higher rate of dead cells compared to the wild-type strain. Virulence towards Caenorhabditis elegans using slow-killing assay was significantly reduced for the mutant compared to the wild-type strain...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Staphylococcus lugdunensis Source Type: research