Release of trivalent chromium from serum transferrin is sufficiently rapid to be physiologically relevant.

Release of trivalent chromium from serum transferrin is sufficiently rapid to be physiologically relevant.
J Inorg Biochem. 2019 Oct 20;202:110901
Authors: Edwards KC, Kim H, Vincent JB
Abstract
Transferrin, the major iron transport protein in the blood, apparently also transports trivalent chromium in vivo via endocytosis. Recent in vitro studies have, however, suggested that the release of chromic ions from serum transferrin is too slow to be biologically relevant. Consequently, the release of chromium(III) from human serum transferrin has been examined under conditions mimicking an endosome during endocytosis. At pH 4.5 and 5.5, the release of Cr(III) from transferrin occurs rapidly from the weak binding site. While appreciably slower, the release of Cr(III) from the tighter site in the presence of biological chelating agents is potentially sufficiently fast to be physiologically relevant. When Cr(III)-loaded transferrin is added to soluble transferrin receptor, the interaction with the receptor results in Cr(III) in both the weak and tight binding sites giving rise to an EPR signal similar to that of the weak binding site; concurrently, the loss of Cr(III) from both binding sites becomes rapid at acidic pH, more rapid than from either site in the absence of the receptor. Loss of Cr(III) from the transferrin-transferrin receptor complex, thus, is easily sufficiently rapid for transferrin to serve as the physiological transporter...

Source: Journal of Inorganic Biochemistry - October 19, 2019 Category: Biochemistry Authors: Edwards KC, Kim H, Vincent JB Tags: J Inorg Biochem Source Type: research