Midkine promotes articular chondrocyte proliferation through the MK-LRP1-nucleolin signaling pathway.

Midkine promotes articular chondrocyte proliferation through the MK-LRP1-nucleolin signaling pathway. Cell Signal. 2019 Oct 19;:109423 Authors: Deng Q, Yu X, Deng S, Ye H, Zhang Y, Han W, Li J, Yu Y Abstract Osteoarthritis (OA) is the most common disease of joint tissues; unfortunately, there are currently no curative therapies available for OA. Chondrocytes, the only cell type residing in cartilage, secrete many types of collagen (the mainly one is type II collagen) and aggrecan, which are the main components of the cartilage matrix. Chondrocyte apoptosis can lead to OA degenerative progression. We previously indicated that recombinant human midkine (rhMK), as a chondrocyte growth factor has a significant reparative effect on cartilage injury animal models. However, the molecular mechanism of this restorative function remains under investigation. Herein, we focused on the molecular mechanism underlying the role of MK in promoting the proliferation of chondrocytes cultured in vitro. Chondrocytes from rats and OA patients were successfully isolated by the digestion of articular cartilage using type II collagenase, and their proliferation was evaluated by a CCK8 assay and flow cytometry. rhMK stimulated the proliferation of chondrocytes from both OA patients and rats. Furthermore, qRT-PCR, shRNA-mediated knockdown, Western blot and immunoprecipitation (IP) assays were performed to identify the receptor and key elements responsible for ...
Source: Cellular Signalling - Category: Cytology Authors: Tags: Cell Signal Source Type: research