Non-lytic M13 phage-based highly sensitive impedimetric cytosensor for detection of coliforms

Publication date: Available online 22 October 2019Source: Biosensors and BioelectronicsAuthor(s): Mohammed Sedki, Xingyu Chen, Chuan Chen, Xin Ge, Ashok MulchandaniAbstractA highly sensitive and selective non-lytic M13 phage-based electrochemical impedance spectroscopy (EIS) cytosensor for early detection of coliforms is introduced for the first time. AuNPs were electrochemically deposited on the surface of glassy carbon electrode, and the M13 phage particles were immobilized on them using 3-mercaptopropanoic acid linker and zero-length crosslinking chemistry (EDC/NHS). Next, the sensor surface was blocked to avoid non-specific binding. The M13-EIS cytosensor was tested for detection of F+ pili Escherichia coli species, using XL1-Blue and K12 strains, as examples of coliforms. The selectivity against non-host strains was demonstrated using Pseudomonas Chlororaphis. The binding of E. coli to the M13 phage on the cytosensor surface increased the charge transfer resistance, enabling detection of coliforms. The biosensor achieved a limit of detection (LOD) of 14 CFU/mL, the lowest reported to-date using EIS-phage sensors, and exhibited a high selectivity towards the tested coliforms. The SEM micrographs confirmed the successful capturing of E. coli on the M13-based EIS cytosensor. Moreover, the sensor showed almost the same sensitivity in the simulated river water samples as in phosphate buffer, reflecting its applicability to real samples. On the other hand, this sensor system...
Source: Biosensors and Bioelectronics - Category: Biotechnology Source Type: research

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Publication date: Available online 12 November 2019Source: Enzyme and Microbial TechnologyAuthor(s): Lovaine Silva Duarte, Laísa Quadros Barsé, Pedro Ferrari Dalberto, William Tadeu Santos da Silva, Rafael Costa Rodrigues, Pablo Machado, Luiz Augusto Basso, Cristiano Valim Bizarro, Marco Antônio Záchia AyubAbstractTransglutaminases (TGases) are a class of transferases widely used in the food and biotechnology industries. In this work, we describe the production of recombinant Bacillus amyloliquefaciens TGase in Escherichia coli, obtaining the protein in its soluble and active form. In order to re...
Source: Enzyme and Microbial Technology - Category: Biotechnology Source Type: research
Conclusions Sevoflurane enhances phagocytosis of bacteria by lipopolysaccharide-challenged macrophagesin vitro andin vivo via an inducible NO synthase –dependent mechanism. Thus, sevoflurane potentiates bactericidal and antiinflammatory host-defense mechanisms in endotoxemia.
Source: Anesthesiology - Category: Anesthesiology Source Type: research
Contributors : V ânia Pobre ; Susana Barahona ; Tatiane Dobrzanski ; Maria B Steffens ; Cecília M ArraianoSeries Type : Expression profiling by high throughput sequencingOrganism : Escherichia coli K-12The transition between exponential and stationary phase is a natural phenomenon for all bacteria and requires a massive readjustment of the bacterial transcriptome. Exoribonucleases are key enzymes in the transition between the two growth phases. PNPase, RNase R and RNase II are the major degradative exoribonucleases in Escherichia coli. We analysed the whole transcriptome of exponential and stationary phases fr...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Escherichia coli K-12 Source Type: research
ConclusionESBL/AmpC-E. coli colonized the gut of travelers to Vietnam with a CTX-resistant E. coli (>107 CFU/g feces), and the colonization lasted 14 weeks.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
In conclusion, mraW was found to be a DNA methylase and have a wide-ranging effect on E. coli O157:H7 including motility and virulence in vivo via genome wide methylation and mraZ antagonism.
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
The quorum sensing (QS) system controls bacterial biofilm formation, which is highly related to the virulence and resistance of pathogens. In the present study, the effect of two traditional Chinese medicine (TCM) monomers, berberine and matrine, on biofilm formation and QS-related gene expression of antimicrobial-resistant (AMR) Escherichia coli strains was investigated by laser scanning confocal microscopy (LSCM) observation and real-time PCR. The results indicated a roughly positive relationship between biofilm formation ability and antimicrobial resistance. LSCM observation showed that berberine and matrine inhibited b...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
The envelope of Gram-negative bacteria is critical for survival across a wide range of environmental conditions. The inner membrane, the periplasm and the outer membrane form a complex compartment, home to many essential processes. Hence, constant monitoring by envelope stress response systems ensure correct biogenesis of the envelope and maintain its homeostasis. Inside the periplasm, the cell wall, made of peptidoglycan, has been under the spotlight for its critical role in bacterial growth as well as being the target of many antibiotics. While much research is centered around understanding the role of the many enzymes i...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
This study showed that the microbiological safety level of street-vended beverages in Chow Kit, Kuala Lumpur was average and needs to be improved. Provision of food safety education and adequate sanitary facilities at vending sites are suggested to increase the safety of food products. 
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Short Communication Source Type: research
This study used indicator kriging (IK) and quantitative microbial risk analysis (QMRA) to assess the health risks of using groundwater for household cleaning and horticultural irrigation on the Pingtung Plain. First, IK was employed to determine the conditional cumulative distribution function (CCDF) of groundwaterEscherichia coli (E. coli). Nonparametric Monte Carlo simulation based on established CCDF was then adopted to characterize the distributions and uncertainty of groundwaterE. coli. Finally, QMRA was employed to determine health risks of groundwater use for household cleaning and horticultural irrigation, and the ...
Source: Environmental Geochemistry and Health - Category: Environmental Health Source Type: research
Authors: Savaş Şen Z, Taşar MA, Demirel Kaya F, Güneş A, Vezir E, Hızarcıoğlu Gülşen H Abstract Cryptosporidium spp. is one of the leading causes of parasitic diarrhea. It is the most common parasite in humans all over the world with Giardia. Cryptosporidium is an important cause of chronic diarrhea in Human Immunodeficiency Virus (HIV)/Acquired Immunodeficiency Syndrome (AIDS) patients. Patients with normal immune system may have an asymptomatic course or clinical presentation such as acute watery diarrhea without blood and persistent diarrhea. The severity and duration of the disease may be a re...
Source: Mikrobiyoloji Bulteni - Category: Microbiology Tags: Mikrobiyol Bul Source Type: research
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