Toxicological evaluation of 3-(4-Chlorophenylselanyl)-1-methyl-1H-indole through the bovine oocyte in vitro maturation model.

Toxicological evaluation of 3-(4-Chlorophenylselanyl)-1-methyl-1H-indole through the bovine oocyte in vitro maturation model.
Toxicol In Vitro. 2019 Oct 17;:104678
Authors: Paschoal JDF, Lopes IA, Borges MA, Feijó AL, Simões LD, Segatto NV, Campos VF, Seixas F, Casaril AM, Savegnago L, Lenardão EJ, Collares T
Abstract
The development of new bioactive molecules based on the molecular hybridization has been widely explored. In line with this, reliable tests should be employed to give information about the toxicology of these new molecules. In this sense, the use of in vitro tests is a valuable tool, especially the in vitro maturation of oocytes (IVM), which is an efficient resource to discover the potential toxicity of synthetic molecules. Thus, the aim of the present study was to evaluate the toxicological effects of the selenium-containing indolyl compound 3-(4-Chlorophenylselanyl)-1-methyl-1H-indole (CMI), on different quality parameters of bovine oocytes through the IVM. Different concentrations of the CMI compound (0, 25, 50, 100, 200 μM) were supplemented during the in vitro maturation process. After, the oocyte maturation rate, glutathione (GSH) levels, reactive oxygen species (ROS) levels, membrane, and mitochondrial integrity were evaluated. The results showed that the lowest concentration of CMI induced the highest GSH production (P < 0.05), an important marker of cytoplasmic quality and maturation. All treatment...

Source: Toxicology in Vitro - October 16, 2019 Category: Toxicology Authors: Paschoal JDF, Lopes IA, Borges MA, Feijó AL, Simões LD, Segatto NV, Campos VF, Seixas F, Casaril AM, Savegnago L, Lenardão EJ, Collares T Tags: Toxicol In Vitro Source Type: research