Generation and characterization of human-derived iPSC lines from three pairs of monozygotic twins discordant for Parkinson's disease

Publication date: Available online 19 October 2019Source: Stem Cell ResearchAuthor(s): Marija Dulovic-Mahlow, Agnieszka Lukomska, Sokhna Haissatou Diaw, Alexander Balck, Max Borsche, Karen Grütz, Insa Lenz, Franziska Rudolph, Katja Lohmann, Christine Klein, Philip SeiblerAbstractDespite a genetic component in the development of Parkinson´s disease (PD), monozygotic twin pairs often display discordance for PD. Here, we describe the generation of six human induced pluripotent stem cell (iPSC) lines from dermal fibroblasts of three pairs of monozygotic twins discordant for PD. We used non-integrating Sendai virus and the iPSC lines were comprehensively characterized. These lines provide a valuable resource for studying molecular differences between the affected and unaffected monozygotic twin and their response to genetic and non-genetic factors that might be involved in the development of PD.
Source: Stem Cell Research - Category: Stem Cells Source Type: research

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Fight Aging! publishes news and commentary relevant to the goal of ending all age-related disease, to be achieved by bringing the mechanisms of aging under the control of modern medicine. This weekly newsletter is sent to thousands of interested subscribers. To subscribe or unsubscribe from the newsletter, please visit: Longevity Industry Consulting Services Reason, the founder of Fight Aging! and Repair Biotechnologies, offers strategic consulting services to investors, entrepreneurs, and others interested in the longevity industry and its complexities. To find out m...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Publication date: Available online 13 November 2019Source: Stem Cell ResearchAuthor(s): Muwan Chen, Muyesier Maimaitili, Susanne Hvolbøl Buchholdt, Uffe Birk Jensen, Fabia Febbraro, Mark DenhamAbstractWe generated eight induced pluripotent stem cell (iPSC) lines from Parkinson's disease (PD) patients with different familial mutations using non-integrating episomal plasmids. All iPSC lines have a normal karyotype, express pluripotent genes including POU5F1, NANOG, and show alkaline phosphatase activity, as well as the ability to differentiate into all three germ layers. These PD iPSC lines can be used for disease mod...
Source: Stem Cell Research - Category: Stem Cells Source Type: research
Innovation may help guide treatment of Alzheimer's, Parkinson's diseases More at This is a Research News item.
Source: NSF Discoveries - Category: Science Source Type: research
Publication date: Available online 9 November 2019Source: Stem Cell ResearchAuthor(s): D.V. Sharipova, V.R. Kovalenko, E.M. Bairamova, V.A. Vartanova, E.V. Grigor'eva, Y.V. Vyatkin, E.A. Khabarova, D.A. Rzaev, S.M. Zakian, S.P. MedvedevAbstractStudying Parkinson's disease (PD), one of the most common neurodegenerative disorders worldwide, requires different model systems, including patient-specific induced pluripotent stem cell lines. With the help of non-integrating episomal vectors the IPSC lines ICGi015-A and ICGi015-B were generated from blood mononuclear cells of PD patient, carrying three SNPs, associated with PD dev...
Source: Stem Cell Research - Category: Stem Cells Source Type: research
Publication date: Available online 6 November 2019Source: Stem Cell ResearchAuthor(s): Seo-Young Lee, Sun-Ku ChungAbstractMutations in leucine-rich repeat kinase 2 (LRRK2) gene (LRRK2 G2019S) is a representative autosomal dominant mutation that can cause Parkinson's disease (PD). A bacterial artificial chromosome-based homologous recombination (BAC-based HR) system was utilized for gene therapy of LRRK2 G2019S-mutant induced pluripotent stem cells (iPSCs) produced by reprogramming episomal vectors. The gene-corrected iPSCs retained typical pluripotency required for their spontaneous differentiation into differentiated cell...
Source: Stem Cell Research - Category: Stem Cells Source Type: research
In conclusion, the present study is the first to report the application of ddPCR in the pharmacokinetics profile description of tracking of hNSCs in preclinical studies. PMID: 31705124 [PubMed - as supplied by publisher]
Source: Acta Pharmacologica Sinica - Category: Drugs & Pharmacology Authors: Tags: Acta Pharmacol Sin Source Type: research
We describe generation of human induced pluripotent stem cell (hiPSC) lines of three unrelated idiopathic late onset Parkinson disease patients and two healthy controls above 60 years of age without neurological diseases nor Ashkenazi ancestry. Human iPSC were derived from peripheral blood-erythroblasts using integration free episomal plasmids carrying four reprogramming factors OCT4, SOX2, c-MYC, KLF4 and BCL-XL. The hiPSC lines were characterized according to established criteria.
Source: Stem Cell Research - Category: Stem Cells Source Type: research
Abstract Parkinson's disease (PD) and frontotemporal dementia/amyotrophic lateral sclerosis (FTD/ALS) are insidious and incurable neurodegenerative diseases that represent a significant burden to affected individuals, caregivers, and an ageing population. Both PD and FTD/ALS are defined at post mortem by the presence of protein aggregates and the loss of specific subsets of neurons. We examine here the crucial role of lysosome dysfunction in these diseases and discuss recent evidence for converging mechanisms. This review draws upon multiple lines of evidence from genetic studies, human tissue, induced pluripotent...
Source: Trends in Neurosciences - Category: Neuroscience Authors: Tags: Trends Neurosci Source Type: research
In this study, we hypothesized that moderately and chronically reducing ACh could attenuate the deleterious effects of aging on NMJs and skeletal muscles. To test this hypothesis, we analyzed NMJs and muscle fibers from heterozygous transgenic mice with reduced expression of the vesicular ACh transporter (VAChT), VKDHet mice, which present with approximately 30% less synaptic ACh compared to control mice. Because ACh is constitutively decreased in VKDHet, we first analyzed developing NMJs and muscle fibers. We found no obvious morphological or molecular differences between NMJs and muscle fibers of VKDHet and contro...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Condition:   Transplantation:Mesenchymal Stem Cell Transplantation Intervention:   Biological: Autologous mesenchymal stem cells Sponsor:   Belarusian Medical Academy of Post-Graduate Education Active, not recruiting
Source: - Category: Research Source Type: clinical trials
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