Analysis of the Uterine Lumen in Fertility-Classified Heifers: I. Glucose, Prostaglandins and Lipids.

Analysis of the Uterine Lumen in Fertility-Classified Heifers: I. Glucose, Prostaglandins and Lipids. Biol Reprod. 2019 Oct 16;: Authors: Moraes JGN, Behura SK, Geary TW, Spencer TE Abstract Survival and growth of the bovine conceptus (embryo and associated extraembryonic membranes) is dependent on endometrial secretions or histotroph found in the uterine lumen. Previously, serial embryo transfer was used to classify heifers as high fertile (HF), subfertile (SF), or infertile (IF). Here, we investigated specific histotroph components (glucose, prostaglandins (PGs) and lipids) in the uterine lumen of day 17 pregnant and open fertility-classified heifers. Concentrations of glucose in the uterine lumen were increased by pregnancy, but did not differ among fertility-classified heifers. Differences in expression of genes encoding glucose transporters and involved with glycolysis and gluconeogenesis were observed between conceptuses collected from HF and SF heifers. In the uterine lumen, PGE2 and PGF2α were increased by pregnancy, and HF heifers had higher concentrations of PGE2, PGF2α and 6-keto-PFG1α than SF heifers. Differences were found in expression of genes regulating PG signaling, arachidonic acid metabolism, and PPAR signaling among conceptuses and endometrium from fertility-classified heifers. Lipidomics was conducted exclusively in samples from HF heifers, and phosphatidylcholine was the main lipid class that increased in the...
Source: Biology of Reproduction - Category: Reproduction Medicine Authors: Tags: Biol Reprod Source Type: research