Using the IPTG-Inducible Pgrac212 Promoter for Overexpression of Human Rhinovirus 3C Protease Fusions in the Cytoplasm of Bacillus subtilis Cells.

In conclusion, the combination of an inducible promoter Pgrac212 and a solubility tag allowed the overexpression of the HRV3C protease in the cytoplasm of B. subtilis. The resulting fusion protein was purified using a nickel column and was active in cleaving target proteins to remove the fusion tags. This study offers an effective method for producing recombinant proteins in the cytoplasm of endotoxin-free bacteria.
PMID: 31612259 [PubMed - as supplied by publisher]

Source: Current Microbiology - October 13, 2019 Category: Microbiology Authors: Le VD, Phan TTP, Nguyen TM, Brunsveld L, Schumann W, Nguyen HD Tags: Curr Microbiol Source Type: research