Biotechnological potential of a cysteine protease (CpCP3) from Calotropis procera latex for cheesemaking
This article reports the characterization and evaluation of the biotechnological potential of a cysteine protease purified from Calotropis procera (CpCP3). This enzyme was highly stable to different metal ions and was able to hydrolyze κ-casein similarly to bovine chymosin. Atomic force microscopy showed that the process of casein micelle aggregation induced by CpCP3 was similar to that caused by chymosin. The cheeses made using CpCP3 showed higher moisture content than those made with chymosin, but protein, fat, and ash were similar. The sensory analysis showed that cheeses made with CpCP3 had high acceptance index (>80%). In silico analysis predicted the presence of only two short allergenic peptides on the surface of CpCP3, which was highly susceptible to digestive enzymes and did not alter zebrafish embryos’ morphology and development. Moreover, recombinant CpCP3 was expressed in Escherichia coli. All results support the biotechnological potential of CpCP3 as an alternative enzyme to chymosin.
Publication date: December 2019Source: Molecular Immunology, Volume 116Author(s): Tomoya Takashima, Tomoki Taku, Tomoka Yamanaka, Tamo Fukamizo, Tomoyuki Numata, Takayuki OhnumaAbstractA 38 kDa β-1,3-glucanase allergen from Cryptomeria japonica pollen (CJP38) was recombinantly produced in E. coli and purified to homogeneity with the use of Ni-affinity resin. CJP38 hydrolyzed β-1,3-glucans such as CM-curdlan and laminarioligosaccharides in an endo-splitting manner. The optimum pH and temperature for β-1,3-glucanase activity were approximately 4.5 and 50 °C, respectively. The enzyme was stabl...
CONCLUSION: The Sperm Agglutinating Factor from Staphylococcus warneri, natural microflora of human cervix, showed extensive potential to be employed as a safe vaginal contraceptive. PMID: 31656198 [PubMed - in process]
A 75-year-old non-atopic male had been suffering from a gastroenteritis-like illness for the last 24 hours characterized by diarrhea and malaise. He decided to take, for the first time, 4 mg of loperamide hydrochloride to assist with symptom relief. Within 30 minutes, he developed severe anaphylaxis with widespread urticaria, presyncope and acute worsening of diarrhea and vomiting. He was transported by ambulance to the hospital, where his initial blood pressure was 82/50. He was successfully resuscitated with two doses of intramuscular epinephrine and intravenous saline.
ConclusionDuring thermal treatment, susceptibility to structural changes of the LTP-fold is highly dependent on the surrounding environment but also on intrinsic features of individual LTPs. This is a crucial fact to consider when processing LTP-containing food or food products as this will directly influence their allergenic potential.
We describe here an infant Treg phenotype characterized by high Treg frequency, the maturation of Treg population characterized by a decrease in their frequency accompanied with an increase in the highly activated Treg cells. These changes in Treg population associated first with the relative abundance of Bifidobacterium longum followed by increasing colonization with butyrate producing bacteria. High bifidobacterial abundance in the neonatal microbiota appeared to be protective, while colonization with Bacteroides and E. coli was associated with later risk of allergy. Estonian children with lower risk of IgE mediated alle...
Recent epidemiological studies suggest that non-IgE-mediated childhood food allergic disorders such as eosinophilic esophagitis (EOE), celiac disease and allergic gastroenteritis (AG), may be increasing in parallel with previously observed increases in childhood IgE-mediated food allergy (FA) and food-induced anaphylaxis (FIA)1-4. The term AG encompasses food protein induced enterocolitis syndrome (FPIES), eosinophilic enteritis and colitis, food protein induced enteropathy, food hypersensitivity enteritis and colitis, but not EOE.
PMID: 31626905 [PubMed - as supplied by publisher]
From 2007 to 2018, the percentage of children aged 0-17 years with a food or digestive allergy in the past 12 months increased from 4.0% in 2007 to 6.5% in 2018.
Abstract Previously, a ubiquinol‑cytochrome c reductase binding protein (UQCRB) homolog was identified in the house dust mite (HDM) species Dermatophagoides farinae (Der f) as a major allergen. In the present study, the immunodominant immunoglobulin E (IgE) epitope of the protein Der f 24 was investigated. Analysis of the homologous amino acid (aa) sequences in Der f and human UQCRB was performed. Four different recombinant Der f 24 and hybrid proteins formed by integrating Der f and human UQCRB sequences were expressed in Escherichia coli, purified using Ni‑NTA resins, and IgE‑binding activity was...
While great strides have been made in diagnosing eosinophilic esophagitis (EoE) over the past decade, the journey to diagnosis of eosinophilic gastrointestinal disorders (EGIDs) below the esophagus remains arduous. EGIDs are named after the location of the gastrointestinal tract that is infiltrated by eosinophils. These include eosinophilic gastritis, enteritis, gastroenteritis and colitis. While the prevalence of EGIDs is currently reported to be around 2-5/100,000, it is possible that it is underestimated given the difficulties many EGID patients face before establishing their diagnosis.