Exploration of the optimized parameters for bioactive prodigiosin mass production and its biomedical applications in vitro as well as in silico

This study explores the optimized conditions for the maximal prodigiosin production, characterization of prodigiosin, biomedical applications against therapeutically important diseases. Prodigiosin was extracted, purified using different solvents and the purified prodigiosin was characterized using FT-IR, UV–Vis Spectrometry and GC-MS analysis. The maximal production of prodigiosin was identified using different optimization methods. UV–Vis spectrophotometry showed the absorbance peak at 530 nm and the FT-IR result revealed prodigiosin contains most of the functional groups that are corresponding to the literature reports and also GC-MS analysis validated the presence of prodigiosin by showing the peak at 323 m/z. Antibacterial activity showed higher activity against Staphylococcus sp. than Gram negative bacteria and showed a very effective activity against Candida sp. than other tested fungi. The larvicidal activity of prodigiosin against Aedes aegypti clearly indicated that 32% and 76% mortality rate was seen at 24 and 48 h respectively. In silico analysis of prodigiosin against Aβ peptide 1–42, a well-known breast cancer protein RAF-1 and two viral proteins E1A and TMV-CP revealed that it has a strong interaction with less binding energy. Altogether, it is strongly endorsed that the prodigiosin pigment could pave a way to treat various bacterial infections and disorders in the forthcoming existences.
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research

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In this study, one bioactive coumarin analog was obtained as a result of biotransformation of three inactive coumarin derivatives by free cells of Candida albicans. The bioactive analog was purified by Column chromatography and HPLC. The presence of coumarin moiety in the biotrasformed product was confirmed by λmax at 350-400 nm and FT-IR spectrum. The structure of the purified compound established by LC-MS and 1H-NMR suggests the chances of biotransformation of 7-(3-(cyclopropylamino)-2-hydroxypropoxy)-4-methyl-2H-chromen-2-one (MW 289 Da) into 7-(3-Cyclopropylamino-2-hydroxy-propoxy)-4-methoxymethyl-...
Source: Process Biochemistry - Category: Biochemistry Source Type: research
Publication date: November 2019Source: Materials Science and Engineering: C, Volume 104Author(s): Abdolhossien Miri, Nafiseh Mahdinejad, Omolbanin Ebrahimy, Mehrdad Khatami, Mina SaraniAbstractZinc oxide nanoparticles (ZnO-NPs) are multifunctional oxide metal nanoparticles that have been considered by many due to their unique properties including UV filtration, semiconducting, antimicrobial, antifungal, and photocatalytic activity. In the present study, ZnO-NPs have been synthesized from Zn(SO4)·7H2O through the use of “green synthesis” method that utilizes Prosopis farcta aqueous extract. The characteri...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
In conclusion, QDs-Cramoll conjugates can be considered effective, specific, and versatile probes able to be applied to evaluate glycan profiles in normal and transformed tissues, by fluorescence microscopy as well as FMA quantification. Furthermore, FMA showed to be a potential method that can be applied with other fluorescent conjugates. PMID: 31306700 [PubMed - as supplied by publisher]
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research
Gulcin Tezcan1, Ekaterina V. Martynova1, Zarema E. Gilazieva1, Alan McIntyre2, Albert A. Rizvanov1 and Svetlana F. Khaiboullina1,3* 1Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia 2Centre for Cancer Sciences, Faculty of Medicine and Health Sciences, University of Nottingham, Nottingham, United Kingdom 3Department of Microbiology and Immunology, University of Nevada, Reno, Reno, NV, United States Inflammation has a crucial role in protection against various pathogens. The inflammasome is an intracellular multiprotein signaling complex that is linked to pathogen sensing and...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
In this study, the three natural compounds 6-methoxymellein (3), angelicoin B (4) and ellagic acid as well as nine novel 3,4-dihydroisocoumarins (Figure 1) were analyzed regarding their cytotoxicity in cancer cells and inhibition of the endogenously expressed human ABC transporters P-gp, BCRP, and MRP1 and of the yeast transporter Pdr5. For further insights into the mechanism of action, Pdr5 ATPase and substrate transport assays were performed. These results were complemented with molecular docking studies that indicate that differences in the inhibitory power of the investigated 3,4-dihydroisocoumarins with respect to P-g...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
In conclusion, this Research Topic includes a wide range of interdisciplinary research work contributing to the ultimate goals of augmenting the existing knowledge and fostering the scientific field of ethnopharmacology in Central and Eastern Europe, as well as globally. This research topic successfully gathered comprehensive interdisciplinary information in the field of ethnopharmacology from the field surveys to standardization and elucidation of molecular mechanisms of action of natural products. Moreover, with consideration of the existing challenges in the field of drug discovery form medicinal plants, this research t...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
This study aimed to contribute to the need of finding new and more effective bioactive molecules against several of the earlier mentioned threats faced by human kind nowadays. For this, eighteen species from the Actinomycetales previously isolated from Erylus spp. sponges collected in Portuguese marine waters (Açores, Madeira, and continental shelf) were screened for antimicrobial, anti-cancer, anti-parasitic and anti-obesogenic activities. Materials and Methods Biological Material The bacteria under study belong to the order Actinomycetales within the phylum Actinobacteria and were isolated from marine sponges o...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
ConclusionOur data revealed significant antimicrobial and antioxidant effects ofC. siliquastrum that contributes significantly to the cytotoxicity of cancer cells that requires further detailed studies.
Source: European Journal of Integrative Medicine - Category: Complementary Medicine Source Type: research
Recent decades have seen improvements in UK breast cancer services and treatments. Despite this, it is recognised that a range of patients ’ psychological and emotional needs remain unmet, both at the time of treatment and into survivorship. Using data from two distinct cohorts of women: those treated with radiotherapy for breast cancer historically, and women treated more recently, this study sought to identify and explore those nee ds and concerns that have continued to persist, despite advances in treatment and care.
Source: European Journal of Oncology Nursing - Category: Cancer & Oncology Authors: Source Type: research
Future Microbiology, Ahead of Print.
Source: Future Microbiology - Category: Microbiology Authors: Source Type: research
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